Papagerakis Petros, Zheng Li, Kim Doohak, Said Raed, Ehlert Amber A, Chung Kevin K M, Papagerakis Silvana
College of Dentistry, University of Saskatchewan, Saskatoon, SK, Canada.
School of Dentistry, University of Michigan, Ann Arbor, MI, USA.
Methods Mol Biol. 2019;1922:549-562. doi: 10.1007/978-1-4939-9012-2_41.
Assaying different biological markers (biomarkers) is commonly used to monitor health status and aid in the diagnosis of diseases. With the recent advances in highly sensitive protein assays, whole saliva (WS) and gingival crevicular fluid (GCF) appear to be fluids that may contain important biomarkers with various applications in dentistry and medicine. Herein, we describe the process of GCF and WS sample collection and preparation for assaying clinically relevant biomarkers in clinical screening trials. Analysis of biomarkers in WS and GCF represents an easy and practical approach for the diagnosis and screening of different pathological conditions particularly in epidemiological surveys.
检测不同的生物标志物常用于监测健康状况并辅助疾病诊断。随着高灵敏度蛋白质检测技术的最新进展，全唾液（WS）和龈沟液（GCF）似乎是可能含有重要生物标志物的体液，在牙科和医学中有各种应用。在此，我们描述了在临床筛查试验中收集和制备GCF和WS样本以检测临床相关生物标志物的过程。分析WS和GCF中的生物标志物是诊断和筛查不同病理状况的一种简单实用的方法，特别是在流行病学调查中。
