Universidade Federal do Ceará, Fortaleza, CE, Brazil.
Food Funct. 2019 Mar 20;10(3):1671-1683. doi: 10.1039/c8fo01575a.
Dietary fiber intake plays an important role in the prevention of obesity. This study aimed at investigating the effect of cashew fiber without low molecular weight compounds (CABwc) on obesity prevention and metabolomics in a murine model of diet-induced obesity. Mice were fed a chow diet (CD), a high-fat diet (HFD) or a high-fat diet supplemented with CABwc (10%) (HFD-CABwc) for 15 weeks. The body weight, abdominal fat, serum glucose levels, insulin and lipid profiles, satiety hormones such as leptin and ghrelin, digestive enzymes such as amylase and lipase, and inflammatory mediators such as TNF-α, IL-6, and adiponectin were measured, in addition to performing serum and hepatic tissue analyses. The metabolomic analysis was based on nuclear magnetic resonance (NMR) spectroscopy of serum and feces. The effects observed with ingestion of CABwc were appetite control and prevention of hyperglycemia, hyperinsulinemia and hypertriglyceridemia, as well as the prevention of the inflammatory process and reduction of liver injury caused by the HFD. In addition, NMR evidenced the presence of SCFAs in serum and feces of mice fed with HFD-CABwc. These findings suggest that CABwc promoted satiety in mice, improving the metabolism of glucose and lipids. Positive effects of obesity prevention may be associated with SCFA production.
膳食纤维的摄入在肥胖预防中起着重要作用。本研究旨在探讨腰果纤维（无低分子量化合物）（CABwc）对饮食诱导肥胖小鼠肥胖预防和代谢组学的影响。小鼠分别用标准饮食（CD）、高脂肪饮食（HFD）或添加 10% CABwc 的高脂肪饮食（HFD-CABwc）喂养 15 周。测量体重、腹部脂肪、血清葡萄糖水平、胰岛素和脂质谱、饱腹感激素（如瘦素和胃饥饿素）、消化酶（如淀粉酶和脂肪酶）以及炎症介质（如 TNF-α、IL-6 和脂联素），此外还进行了血清和肝组织分析。代谢组学分析基于血清和粪便的核磁共振（NMR）光谱。摄入 CABwc 的效果表现为控制食欲和预防高血糖、高胰岛素血症和高三酰甘油血症，以及预防 HFD 引起的炎症过程和减轻肝损伤。此外，NMR 证明了 HFD-CABwc 喂养的小鼠血清和粪便中存在短链脂肪酸（SCFAs）。这些发现表明 CABwc 促进了小鼠的饱腹感，改善了葡萄糖和脂质的代谢。肥胖预防的积极影响可能与 SCFA 的产生有关。
