Sallent Ignacio, Capella-Monsonís Héctor, Zeugolis Dimitrios I
Regenerative, Modular and Developmental Engineering Laboratory (REMODEL), National University of Ireland Galway (NUI Galway), Galway, Ireland.
Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM), National University of Ireland Galway (NUI Galway), Galway, Ireland.
Methods Mol Biol. 2019;1944:23-38. doi: 10.1007/978-1-4939-9095-5_3.
Chemical cross-linking of collagen-based devices is used as a means of increasing the mechanical stability and control the degradation rate upon implantation. Herein, we describe techniques to produce cross-linked with glutaraldehyde (GTA; amine terminal cross-linker), 4-arm polyethylene glycol succinimidyl glutarate (4SP; amine terminal cross-linker), diphenyl phosphoryl azide (DPPA; carboxyl terminal cross-linker), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC; carboxyl terminal cross-linker) collagen films. In addition, we provide protocols to characterize the biophysical (swelling), biomechanical (tensile), and biological (metabolic activity, proliferation and viability using human dermal fibroblasts and THP-1 macrophages) properties of the cross-linked collagen scaffolds.
基于胶原蛋白的装置的化学交联被用作提高机械稳定性和控制植入后降解速率的一种手段。在此，我们描述了用戊二醛（GTA；胺末端交联剂）、4-臂聚乙二醇琥珀酰亚胺戊二酸酯（4SP；胺末端交联剂）、二苯基磷酰叠氮化物（DPPA；羧基末端交联剂）和1-乙基-3-(3-二甲基氨基丙基)碳二亚胺（EDC；羧基末端交联剂）制备交联胶原蛋白膜的技术。此外，我们还提供了表征交联胶原蛋白支架的生物物理（膨胀）、生物力学（拉伸）和生物学（使用人真皮成纤维细胞和THP-1巨噬细胞的代谢活性、增殖和活力）特性的方案。
