Institut für Chemie und Biochemie , Freie Universität Berlin , Takustr. 3 , 14195 Berlin , Germany.
Department of Chemistry, Faculty of Science , Lorestan University , Khorram Abad , Iran.
Langmuir. 2019 Apr 2;35(13):4736-4746. doi: 10.1021/acs.langmuir.8b03660. Epub 2019 Mar 19.
An understanding of the interactions of 2D nanomaterials with pathogens is of vital importance to developing and controlling their antimicrobial properties. In this work, the interaction of functionalized graphene with tunable hydrophobicity and bacteria is investigated. Poly(ethylene glycol)- block-(poly- N-isopropylacrylamide) copolymer (PEG- b-PNIPAM) with the triazine joint point was attached to the graphene surface by a nitrene [2 + 1] cycloaddition reaction. By thermally switching between hydrophobic and hydrophilic states, functionalized graphene sheets were able to bind to bacteria. Bacteria were eventually disrupted when the functionality was switched to the hydrophobic state. On the basis of measuring the different microscopy methods and a live/dead viability assay, it was found that Escherichia coli ( E. coli) bacteria are more susceptible to hydrophobic interactions than B. cereus bacteria, under the same conditions. Our investigations confirm that hydrophobic interaction is one of the main driving forces at the presented graphene/bacteria interfaces and promotes the antibacterial activity of graphene derivatives significantly.
理解 2D 纳米材料与病原体的相互作用对于开发和控制它们的抗菌性能至关重要。在这项工作中，研究了具有可调疏水性的功能化石墨烯与细菌的相互作用。通过氮宾 [2 + 1] 环加成反应，将具有三嗪连接点的聚（乙二醇）-嵌段-聚（N-异丙基丙烯酰胺）共聚物（PEG-b-PNIPAM）接枝到石墨烯表面。通过在疏水性和亲水性状态之间进行热切换，功能化的石墨烯片能够与细菌结合。当功能切换到疏水性状态时，细菌最终被破坏。基于不同显微镜方法和活/死活力测定的测量，发现在相同条件下，大肠杆菌（E. coli）细菌比蜡状芽孢杆菌（B. cereus）细菌更容易受到疏水性相互作用的影响。我们的研究证实，疏水性相互作用是所提出的石墨烯/细菌界面的主要驱动力之一，并显著促进了石墨烯衍生物的抗菌活性。
