Lee Hyun-Ju, Sung Jae Hoon, Hong Jae Taek, Kim Il Sup, Yang Seung Ho, Cho Chul Bum
Department of Science in Korean Medicine, Graduate School, College of Korean Medicine, Kyung Hee University, Seoul, Korea.
Department of Neurosurgery, St. Vincent's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
J Korean Neurosurg Soc. 2019 Mar;62(2):166-174. doi: 10.3340/jkns.2018.0122. Epub 2019 Feb 27.
Globus pallidus interna (GPi) is acknowledged as an essential treatment for advanced Parkinson's disease (PD). Nonetheless, the neurotransmitter study about its results is undiscovered. The goal of this research was to examine influences of entopeduncular nucleus (EPN) stimulation, identical to human GPi, in no-lesioned (NL) rat and 6-hydroxydopamine (6-HD)-lesioned rat on glutamate change in the striatum.
Extracellular glutamate level changes in striatum of NL category, NL with deep brain stimulation (DBS) category, 6-HD category, and 6-HD with DBS category were examined using microdialysis and high-pressure liquid chromatography. Tyrosine hydroxylase (TH) immunoreactivities in substantia nigra and striatum of the four categories were also analyzed.
Extracellular glutamate levels in the striatum of NL with DBS category and 6-HD with DBS category were significantly increased by EPN stimulation compared to those in the NL category and 6-HD category. EPN stimulation had no significant effect on the expression of TH in NL or 6-HD category.
Clinical results of GPi DBS are not only limited to direct inhibitory outflow to thalamus. They also include extensive alteration within basal ganglia.
苍白球内侧部（GPi）被公认为是晚期帕金森病（PD）的重要治疗靶点。然而，关于其治疗效果的神经递质研究尚未见报道。本研究旨在探讨与人类GPi相同的内苍白球核（EPN）刺激对未损伤（NL）大鼠和6-羟基多巴胺（6-HD）损伤大鼠纹状体谷氨酸变化的影响。
采用微透析和高压液相色谱法检测NL组、NL伴深部脑刺激（DBS）组、6-HD组和6-HD伴DBS组大鼠纹状体细胞外谷氨酸水平的变化。同时分析四组大鼠黑质和纹状体中酪氨酸羟化酶（TH）的免疫反应性。
与NL组和6-HD组相比，EPN刺激显著增加了NL伴DBS组和6-HD伴DBS组大鼠纹状体细胞外谷氨酸水平。EPN刺激对NL组或6-HD组TH的表达无显著影响。
GPi DBS的临床效果不仅限于对丘脑的直接抑制性输出。还包括基底神经节内的广泛改变。
