Bull World Health Organ. 1978;56(3):467-80.
Studies on the characterization of salivarian trypanosomes are at present developing rapidly and difficulties in communication tend to arise because of different procedures in the different laboratories concerned. Terms can be divided into two classes, those that are purely operational and simply describe the laboratory history of the organism, and those that imply characterization. Of the Linnaean taxa, the section Salivaria and the four subgenera thereof, Duttonella, Nannomonas, Pycnomonas, and Trypanozoon, are useful at the present time but lower taxa are often unsatisfactorily defined. Characters such as the clinical course of infection, which have been used for subspeciation, are being found to correlate with such characters as isoenzyme patterns, antigenic make-up and DNA constitution.Serial passage of organisms has serious disadvantages and so reference collections are primarily of stabilates-cryopreserved suspensions of living organisms. Methods for the preparation of antigens, antisera, and isoenzymes are briefly noted.Conventions for the documentation of reference collections are proposed, in particular that the designation of materials should consist of two components, one signifying the primary isolation and the other the particular derived material used. The requirements for rapid information retrieval and global cataloguing of materials are considered.
目前，关于涎源性锥虫特征的研究发展迅速，由于相关不同实验室采用不同程序，交流中往往会出现困难。术语可分为两类，一类是纯粹操作性的，仅描述生物体的实验室记录，另一类则意味着特征描述。在林奈分类单元中，涎源亚目及其四个亚属，即达顿锥虫属、小锥虫属、密锥虫属和锥虫亚属，目前是有用的，但较低分类单元的定义往往不尽人意。曾用于亚种划分的感染临床过程等特征，正被发现与同工酶模式、抗原组成和DNA构成等特征相关。生物体的连续传代有严重缺点，因此参考标本主要是稳定株——生物体的冷冻保存悬液。简要介绍了抗原、抗血清和同工酶的制备方法。提出了参考标本记录规范，特别是材料的命名应由两部分组成，一部分表示初次分离，另一部分表示所使用的特定衍生材料。考虑了快速信息检索和材料全球编目的要求。
