1 Department of Biomedical Engineering, Purdue School of Engineering and Technology Indiana University-Purdue University at Indianapolis.
2 Department of Medical Equipment Technology, College of Applied Medical Science Majmaah University, Almajmaah, Riyadh, Saudi Arabia.
J Biomater Appl. 2019 May;33(10):1415-1426. doi: 10.1177/0885328219834680. Epub 2019 Mar 6.
This study reports synthesis and attachment of a novel antibacterial and hydrophilic polymer onto a polyvinylchloride surface via a simple and mild surface coating technique. The compound 3,4-dichloro-5-hydroxy-2(5H)-furanone was derivatized and copolymerized with N-vinylpyrrolidone. The copolymer was then covalently coated onto polyvinylchloride surface. 3T3 mouse fibroblast cells and bacterium Pseudomonas aeruginosa were used to evaluate surface adhesion and antibacterial activity. Results showed that the polymer-modified polyvinylchloride surface not only exhibited significantly decreased 3T3 fibroblast cell adhesion with a 64-84% reduction but also demonstrated significantly decreased P. aeruginosa adhesion with a 65-84% reduction, as compared to unmodified polyvinylchloride. Furthermore, the modified polyvinylchloride surfaces exhibited significant antibacterial functions by inhibiting P. aeruginosa growth with a 58-80% reduction and killing bacteria, as compared to unmodified polyvinylchloride. These results demonstrate that covalent polymer attachment conferred cell/bacterial adhesion-resistant and antibacterial properties to the polyvinylchloride surface.
本研究通过简单温和的表面涂覆技术，将一种新型抗菌亲水性聚合物附着到聚氯乙烯表面。将 3,4-二氯-5-羟基-2(5H)-呋喃酮衍生化并与 N-乙烯基吡咯烷酮共聚，然后将共聚物共价涂覆到聚氯乙烯表面。用 3T3 小鼠成纤维细胞和细菌铜绿假单胞菌来评估表面黏附性和抗菌活性。结果表明，与未改性的聚氯乙烯相比，聚合物改性的聚氯乙烯表面不仅显著降低了 3T3 成纤维细胞黏附，减少了 64%-84%，而且还显著降低了铜绿假单胞菌黏附，减少了 65%-84%。此外，与未改性的聚氯乙烯相比，改性的聚氯乙烯表面通过抑制铜绿假单胞菌生长（减少 58%-80%）和杀死细菌，表现出显著的抗菌功能。这些结果表明，共价聚合物附着赋予了聚氯乙烯表面抗细胞/细菌黏附性和抗菌性。
