Mycobacterium Research Laboratory, Rajiv Gandhi Centre for Biotechnology, Thycaud P.O., Thiruvananthapuram, 695014, India.
Sci Rep. 2019 Mar 6;9(1):3660. doi: 10.1038/s41598-019-40051-5.
Mycobacterium tuberculosis employs several strategies to combat and adapt to adverse conditions encountered inside the host. The non-replicative dormant state of the bacterium is linked to drug resistance and slower response to anti-tubercular therapy. It is known that alterations in lipid content allow dormant bacteria to acclimatize to cellular stress. Employing comparative lipidomic analysis we profiled the changes in lipid metabolism in M. tuberculosis using a modified Wayne's model of hypoxia-induced dormancy. Further we subjected the dormant bacteria to resuscitation, and analyzed their lipidomes until the lipid profile was similar to that of normoxially grown bacteria. An enhanced degradation of cell wall-associated and cytoplasmic lipids during dormancy, and their gradual restoration during reactivation, were clearly evident. This study throws light on distinct lipid metabolic patterns that M. tuberculosis undergoes to maintain its cellular energetics during dormancy and reactivation.
结核分枝杆菌采用多种策略来应对和适应宿主内遇到的不利条件。细菌的非复制休眠状态与耐药性和对抗结核治疗的反应较慢有关。已知脂质含量的改变使休眠细菌能够适应细胞应激。通过使用改良的韦恩缺氧诱导休眠模型，我们进行了比较脂质组学分析，以描绘分枝杆菌脂质代谢的变化。进一步，我们使休眠细菌复苏，并分析它们的脂质组，直到脂质图谱与常氧生长的细菌相似。在休眠期间，细胞壁相关和细胞质脂质的降解明显增强，在复苏期间逐渐恢复。这项研究揭示了结核分枝杆菌在休眠和复苏过程中维持细胞能量代谢所经历的独特脂质代谢模式。
