Chaire de Recherche industrielle du CRSNG en salubrité des viandes, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, Canada.
Groupe de recherche et d'enseignement en salubrité alimentaire, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, Canada.
PLoS One. 2019 Mar 7;14(3):e0212946. doi: 10.1371/journal.pone.0212946. eCollection 2019.
Campylobacter jejuni is a zoonotic agent responsible for the foodborne gastroenteritis campylobacteriosis. Control of C. jejuni load in the poultry primary production is recognized as an avenue to reduce human exposure to the pathogen. As for now, no commercially applicable control methods exist at the farm. Several studies tested egg yolk powders, potentiated or not against C. jejuni, as feed additives for chicken and suggested that the quantity and quality of the antibodies presence in the yolk are determinant factors for the full success of this approach. Unfortunately, data from these studies inconsistently showed a reduction of cecal C. jejuni carriage. Our first goal wwas to characterize (quantification by ELISA, agglutination test, bacterial antigen recognition profiles by Western blot, bactericidal effect by serum killing assays and C. jejuni mobility by soft agar migation) the antibodies extracted from egg yolk powders originating from different egg production protocols. Secondly, these powders were microencapsulated and recharacterized. Finally the protected powders were tested as a feed additive to destabilize C. jejuni colonization in an in vivo assay. Despite the in vitro results indicating the ability of the egg yolk powders to recognize Campylobacter and potentially alter its colonization of the chicken caecum, these results were not confirmed in the in vivo trial despite that specific caecal IgY directed toward Campylobacter were detected in the groups receiving the protected powders. More research is needed on Campylobacter in order to effectively control this pathogen at the farm.
空肠弯曲菌是一种动物源性病原体，可引起食源性胃肠炎——弯曲菌病。控制家禽初级生产中弯曲菌的负荷被认为是减少人类接触病原体的途径。到目前为止，农场还没有商业上可应用的控制方法。一些研究测试了蛋黄粉，不论是否对弯曲菌有增效作用，作为鸡的饲料添加剂，并表明蛋黄中存在的抗体数量和质量是该方法完全成功的决定因素。不幸的是，这些研究的数据不一致地显示了盲肠弯曲菌携带量的减少。我们的第一个目标是从不同的鸡蛋生产方案中提取的蛋黄粉中提取的抗体进行特性分析（通过 ELISA、凝集试验进行定量、Western blot 进行细菌抗原识别谱分析、血清杀菌试验进行杀菌效果分析和软琼脂迁移试验进行 C. jejuni 迁移分析）。其次，对这些粉末进行微囊化并重新进行特性分析。最后，将保护后的粉末作为饲料添加剂进行测试，以在体内试验中破坏 C. jejuni 的定植。尽管体外结果表明蛋黄粉能够识别弯曲菌并可能改变其对鸡盲肠的定植，但在体内试验中并未得到证实，尽管在接受保护后的粉末的组中检测到针对弯曲菌的特异性盲肠 IgY。为了有效地在农场控制这种病原体，还需要对弯曲菌进行更多的研究。
