Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B9820, Merelbeke, Belgium.
Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B9820, Merelbeke, Belgium.
Sci Rep. 2019 Jun 20;9(1):8931. doi: 10.1038/s41598-019-45380-z.
Campylobacter infections sourced mainly to poultry products, are the most important bacterial foodborne zoonoses worldwide. No effective measures to control these infections in broiler production exist to date. Here, we used passive immunization with hyperimmune egg yolks to confer broad protection of broilers against Campylobacter infection. Two novel vaccines, a bacterin of thirteen Campylobacter jejuni (C. jejuni) and C. coli strains and a subunit vaccine of six immunodominant Campylobacter antigens, were used for the immunization of layers, resulting in high and prolonged levels of specific immunoglobulin Y (IgY) in the hens' yolks. In the first in vivo trial, yolks (sham, bacterin or subunit vaccine derived) were administered prophylactically in the broiler feed. Both the bacterin- and subunit vaccine-induced IgY significantly reduced the number of Campylobacter-colonized broilers. In the second in vivo trial, the yolks were administered therapeutically during three days before euthanasia. The bacterin IgY resulted in a significant decrease in C. jejuni counts per infected bird. The hyperimmune yolks showed strong reactivity to a broad representation of C. jejuni and C. coli clonal complexes. These results indicate that passive immunization with hyperimmune yolks, especially bacterin derived, offers possibilities to control Campylobacter colonization in poultry.
空肠弯曲菌感染主要来源于家禽产品，是全球最重要的细菌性食源性人畜共患病。迄今为止，尚无有效的措施来控制肉鸡生产中的这些感染。在这里，我们使用高免蛋黄进行被动免疫，为肉鸡提供针对弯曲菌感染的广泛保护。两种新型疫苗，一种是 13 株空肠弯曲菌（C. jejuni）和大肠杆菌的菌苗，另一种是 6 种免疫优势弯曲菌抗原的亚单位疫苗，用于对蛋鸡进行免疫接种，导致母鸡蛋黄中特异性免疫球蛋白 Y（IgY）水平升高并持续时间延长。在第一次体内试验中，蛋黄（假对照、菌苗或亚单位疫苗）被预防性地添加到肉鸡饲料中。菌苗和亚单位疫苗诱导的 IgY 均显著减少了弯曲菌定植的肉鸡数量。在第二次体内试验中，蛋黄在安乐死前三天进行治疗性给药。菌苗 IgY 导致每只感染鸡的空肠弯曲菌数量显著减少。高免蛋黄对广泛代表性的空肠弯曲菌和大肠杆菌克隆复合体具有强烈的反应性。这些结果表明，用高免蛋黄进行被动免疫，特别是用菌苗进行被动免疫，为控制家禽中的弯曲菌定植提供了可能。
