Department of Agricultural Sciences, University of Bologna, viale Fanin 44, 40127 Bologna, Italy.
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', National Reference Laboratory for Campylobacter, Via Campo Boario, 64100 Teramo, Italy.
Int J Food Microbiol. 2017 Jun 19;251:41-47. doi: 10.1016/j.ijfoodmicro.2017.04.001. Epub 2017 Apr 3.
C. jejuni is considered a food safety concern to both public health authorities and consumers since it is the leading bacterial cause of food-borne gastroenteritis in humans. A high incidence of C. jejuni in broiler flocks is often correlated to pathogen recovery in retail poultry meat, which is the main source of human infection. In this work broiler chickens were fed with a synbiotic product mixed with conventional feed using two different administration strategies. The synbiotic was formulated with the microencapsulated probiotic Bifidobacterium longum PCB133 and a xylo-oligosaccharide (XOS). 1-day old chicks were infected with C. jejuni strain M1 (10 cells) and the synbiotic mixture was then administered starting from the first and the 14th day of chicken life (for animal groups GrpC and GrpB respectively). The goal of this study was to monitor C. jejuni load at caecum level at different sampling time by real-time PCR, identifying the best administration strategy. The microbiological analysis of the caecal content also considered the quantification of Campylobacter spp., Bifidobacterium spp. and B. longum. The supplemented synbiotic was more successful in reducing C. jejuni and Campylobacter spp. when administered lifelong, compared to the shorter supplementation (GrpB). Bifidobacterium spp. quantification did not show significant differences among treatments and B. longum PCB133 was detected in both supplemented groups evidencing the successful colonization of the strain. Moreover, the samples of the control group (GrpA) and GrpC were analysed with PCR-denaturing gradient gel electrophoresis (PCR-DGGE) to compare the caecal microbial community profiles at the beginning and at the end of the trial. Pattern analysis evidenced the strong influence of the early synbiotic supplementation, although a physiological change in the microbial community, occurring during growth, could be observed. Experimental results demonstrate that the synbiotic approach at farm level can be an effective strategy, combined with biosecurity measures, to improve the safety of poultry meat.
空肠弯曲菌被认为是公共卫生当局和消费者都关注的食品安全问题，因为它是人类食源性胃肠炎的主要细菌病原体。肉鸡群中空肠弯曲菌的高发率通常与零售禽肉中病原体的恢复有关，而零售禽肉是人类感染的主要来源。在这项工作中，肉鸡用含有微囊化益生菌长双歧杆菌 PCB133 和木二糖（XOS）的合生元产品与常规饲料混合喂养，采用两种不同的给药策略。1 日龄小鸡感染空肠弯曲菌 M1 株（10 个细胞），然后从鸡生命的第 1 天和第 14 天开始（分别对应动物组 GrpC 和 GrpB）给予合生元混合物。本研究的目的是通过实时 PCR 监测不同时间点盲肠水平的空肠弯曲菌负荷，确定最佳给药策略。盲肠内容物的微生物分析还考虑了弯曲菌属、双歧杆菌属和长双歧杆菌的定量。当终生给予补充合生元时，与较短时间的补充（GrpB）相比，它更成功地降低了空肠弯曲菌和弯曲菌属的数量。补充双歧杆菌属的数量在处理之间没有显示出显著差异，并且在两个补充组中都检测到了长双歧杆菌 PCB133，证明了该菌株的成功定植。此外，对对照组（GrpA）和 GrpC 的样本进行了聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）分析，以比较试验开始和结束时盲肠微生物群落的图谱。模式分析证明了早期合生元补充的强烈影响，尽管在生长过程中可以观察到微生物群落的生理变化。实验结果表明，在农场水平上采用合生元方法结合生物安全措施是提高禽肉安全性的有效策略。
