STAR Unit of Renal Biochemistry and Stone Disease, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
PLoS One. 2019 Mar 7;14(3):e0213180. doi: 10.1371/journal.pone.0213180. eCollection 2019.
Familial members of urolithiasis have high risk for stone development. We observed the low sulfated glycosaminoglycan (GAG) excretion in urolithiasis patients and their descendants. In this study, we investigated urinary excretion of sulfated GAG, chondroitin sulfate (CS), heparan sulfate (HS) and hyaluronic acid (HA) in urolithiasis and their children, and explored the effect of CS and HA supplement in urolithic hyperoxaluric rats. The 24-hour urines were collected from urolithiasis patients (28) and their children (40), as well as healthy controls (45) and their children (33) to measure urinary sulfated GAG, CS, HS and HA excretion rate. Our result showed that urinary sulfated GAG and CS were diminished in both urolithiasis patients and their children, while decreased HS and increased HA were observed only in urolithiasis patients. Percentage of HS per sulfated GAG increased in both urolithiasis patients and their children. In hyperoxaluric rats induced by ethylene glycol and vitamin D, we found that CS supplement could prevent stone formation, while HA supplement had no effect on stone formation. Our study revealed that decreased urinary GAG and CS excretion are common in familial members of urolithiasis patients, and CS supplement might be beneficial in calcium oxalate urolithiasis prophylaxis for hyperoxaluric patients.
结石病患者的家族成员结石发病风险高。我们观察到结石病患者及其后代的硫酸化糖胺聚糖 (GAG) 排泄量较低。在这项研究中，我们研究了结石病患者及其子女的尿硫酸化 GAG、硫酸软骨素 (CS)、硫酸乙酰肝素 (HS) 和透明质酸 (HA) 的排泄情况，并探讨了 CS 和 HA 补充对草酸钙结石高草酸尿症大鼠的影响。收集了 28 名结石病患者和他们的 40 名子女、45 名健康对照者及其 33 名子女的 24 小时尿液，以测量尿硫酸化 GAG、CS、HS 和 HA 的排泄率。我们的结果表明，结石病患者及其子女的尿硫酸化 GAG 和 CS 均减少，而仅在结石病患者中观察到 HS 减少和 HA 增加。硫酸化 GAG 中的 HS 百分比在结石病患者及其子女中均增加。在乙二醇和维生素 D 诱导的高草酸尿症大鼠中，我们发现 CS 补充可预防结石形成，而 HA 补充对结石形成没有影响。我们的研究表明，结石病患者家族成员中普遍存在尿 GAG 和 CS 排泄减少的现象，CS 补充可能有益于高草酸尿症患者的草酸钙结石预防。
