Pozzer L, Bezerra I C, Kormelink R, Prins M, Peters D, Resende R de O, de Ávila A C
Embrapa/Hortaliças, Km 09, Rodovia Brasília Anápolis, CEP: 70.359-970, Brasília, DF, Brazil.
Wageningen Agricultural University, Laboratory of Virology, Binnenhaven 11, 6709 PD, Wageningen, The Netherlands.
Plant Dis. 1999 Apr;83(4):345-350. doi: 10.1094/PDIS.1999.83.4.345.
A tospovirus from onion causing a disease known as "sapeca" by growers in Brazil was characterized. Symptoms on onion consisted of numerous eyelike spots on the leaves and flower stalks resulting in flower abortion. Nicotiana benthamiana and N. rustica were the only systemic hosts experimentally found. Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) experiments demonstrated that this virus was serologically related to iris yellow spot virus (IYSV), a tospovirus recently described in the Netherlands. This virus, from onion, based on an amino acid sequence identity of 90.5% for the N gene protein, is regarded as a strain of IYSV and is designated IYSV This 10% divergence in the nucleocapsid protein may represent an adaptation of the virus to distinct ecological niches.
对一种来自洋葱的番茄斑萎病毒属病毒进行了特性鉴定，巴西种植者称这种病害为“萨佩卡”。洋葱上的症状表现为叶片和花茎上出现大量眼状斑点，导致花朵败育。实验发现，本氏烟草和黄花烟草是仅有的系统寄主。双抗体夹心酶联免疫吸附测定（DAS-ELISA）实验表明，该病毒与荷兰最近描述的一种番茄斑萎病毒属病毒——鸢尾黄斑病毒（IYSV）存在血清学关系。基于N基因蛋白90.5%的氨基酸序列同一性，这种来自洋葱的病毒被视为IYSV的一个株系，并被命名为IYSV。核衣壳蛋白中这10%的差异可能代表了该病毒对不同生态位的适应性。
