Geng Fengxue, Wang Qingxuan, Li Chen, Liu Junchao, Zhang Dongmei, Zhang Shuwei, Pan Yaping
Department of Periodontics, School of Stomatology, China Medical University, Shenyang, China.
State Key Laboratory of Oral Disease, School of Stomatology, Sichuan University, Chengdu, China.
Front Oncol. 2019 Feb 21;9:91. doi: 10.3389/fonc.2019.00091. eCollection 2019.
Recent investigations revealed the relationship between chronic periodontitis, and cancer. However, host genes that change in response to chronic infection with and may contribute to oral cancer have remained largely unknown. In the present study, we aimed to comprehensively analyze microarray data obtained from the chronic infection model of immortalized oral epithelial cells that were persistently exposed to for 15 weeks. Using protein-protein interaction (PPI) networks and Ingenuity Pathway Analysis (IPA), we identified hub genes, major biological processes, upstream regulators and genes potentially involved in tumor initiation and progression. We also validated gene expression and demonstrated genetic alteration of hub genes from clinical samples of head and neck cancer. Overall, we utilized bioinformatical methods to identify , and as potential candidate genes that might facilitate the prevention and treatment of oral squamous cell carcinoma (OSCC), the most common type of head and neck squamous cell carcinoma (HNSCC).
近期研究揭示了慢性牙周炎与癌症之间的关系。然而，宿主基因在应对慢性感染时发生变化并可能导致口腔癌，这一点在很大程度上仍不为人所知。在本研究中，我们旨在全面分析从永生化口腔上皮细胞慢性感染模型中获得的微阵列数据，这些细胞持续暴露于[具体物质]15周。利用蛋白质-蛋白质相互作用（PPI）网络和 Ingenuity 通路分析（IPA），我们确定了枢纽基因、主要生物学过程、上游调节因子以及可能参与肿瘤起始和进展的基因。我们还验证了基因表达，并展示了头颈癌临床样本中枢纽基因的基因改变。总体而言，我们利用生物信息学方法确定了[具体基因]作为潜在候选基因，这些基因可能有助于预防和治疗口腔鳞状细胞癌（OSCC），这是头颈鳞状细胞癌（HNSCC）最常见的类型。
