Dewanjee M K, Wahner H W, Dunn W L, Robertson J S, Offord K P, Fuster V D, Chesebro J H
Mayo Clin Proc. 1986 May;61(5):327-36. doi: 10.1016/s0025-6196(12)61949-1.
We studied mean platelet survival times in healthy volunteers with use of [51Cr]disodium chromate, 111In-oxine (in a solution of acid-citrate-dextrose [ACD] and saline), and 111In-tropolone (in ACD-plasma) as markers. Differences found between the 51Cr and 111In labels probably can be attributed to a variation in localization of the label on the cell and of renal handling of the free label after release. The mean platelet survival time with 51Cr was slightly longer than the survival time with both indium labels and showed a sex difference not seen with 111In-oxine. Protein-bound plasma 51Cr was lower than plasma 111In and remained constant throughout the study. Plasma 111In increased with time. For survival time calculations, no correction for free 51Cr is necessary, but correction should routinely be performed when 111In markers are used. Both 111In markers gave similar results with respect to platelet survival time. The somewhat more elaborate plasma labeling procedure with tropolone shows no measurable advantage over the original 111In-oxine method. 111In-tropolone labeling takes less time and maintains platelets in the physiologic environment of plasma during incubation. Biodistribution studies show no difference between the two 111In markers.
我们使用[51Cr]铬酸钠、111铟-奥克辛（在酸-柠檬酸-葡萄糖[ACD]和生理盐水溶液中）以及111铟-托酚酮（在ACD-血浆中）作为标记物，研究了健康志愿者的平均血小板存活时间。51Cr和111铟标记物之间发现的差异可能归因于标记物在细胞上的定位变化以及释放后游离标记物的肾脏处理差异。51Cr标记的平均血小板存活时间略长于两种铟标记物的存活时间，并且显示出111铟-奥克辛未观察到的性别差异。与血浆111铟相比，蛋白结合的血浆51Cr较低，并且在整个研究过程中保持恒定。血浆111铟随时间增加。对于存活时间计算，无需对游离51Cr进行校正，但使用111铟标记物时应常规进行校正。两种111铟标记物在血小板存活时间方面给出了相似的结果。使用托酚酮的血浆标记程序略显复杂，但与原始的111铟-奥克辛方法相比没有可测量的优势。111铟-托酚酮标记所需时间更少，并且在孵育期间将血小板维持在血浆的生理环境中。生物分布研究表明两种111铟标记物之间没有差异。
