Aquatic Ecology and Water Quality Management Group, Wageningen University, P.O. Box 47, 6700 AA, Wageningen, the Netherlands.
Aquatic Ecology and Water Quality Management Group, Wageningen University, P.O. Box 47, 6700 AA, Wageningen, the Netherlands.
Environ Pollut. 2019 May;248:676-683. doi: 10.1016/j.envpol.2019.02.061. Epub 2019 Feb 25.
The response of sediment bacterial communities in subtropical freshwater benthic microcosms to sediment-associated triclosan (TCS; 28 d exposure) was analysed using Illumina high-throughput sequencing. This study highlights the interactive effects of TCS and the presence of benthic macroinvertebrates (Limnodrilus hoffmeisteri and Viviparidae bellamya) on sediment bacterial communities. Our results show that TCS alone significantly altered the taxonomic composition and decreased alpha diversity of sediment bacterial communities at concentrations ≥80 μg TCS/g dry weight (dw) sediment (sed). Regarding dominant phyla, TCS significantly reduced the relative abundance of Bacteroidetes and Firmicutes at these concentrations, whereas the relative abundance of Chloroflexi and Cyanobacteria increased. In the presence of benthic macroinvertebrates, the sediment bacterial community was affected by 8 μg TCS/g dw sed as well. However, the presence of benthic macroinvertebrates did not cause measurable changes to bacterial community in unspiked (i.e., control) sediment. These results indicate that TCS alone would not alter the sediment bacterial community at environmentally relevant concentrations (up till 8 μg/g dw sed), but may have an effect in combination with the presence of benthic macroinvertebrates. Therefore, we recommend to include benthic macroinvertebrates when assessing the response of sediment bacterial communities during exposure to environmental stress such as organic contaminants.
本研究采用 Illumina 高通量测序技术分析了亚热带淡水底栖微宇宙中沉积物细菌群落对与沉积物结合的三氯生（TCS；28d 暴露）的响应。这项研究强调了 TCS 与底栖大型无脊椎动物（霍夫迈斯特环毛蚓和圆田螺科）的存在对沉积物细菌群落的相互作用影响。我们的结果表明，TCS 单独作用在浓度≥80μg TCS/g 干重（dw）沉积物（sed）时，会显著改变沉积物细菌群落的分类组成和降低 alpha 多样性。就主要门而言，在这些浓度下，TCS 显著降低了拟杆菌门和厚壁菌门的相对丰度，而绿弯菌门和蓝藻门的相对丰度增加。在底栖大型无脊椎动物存在的情况下，sed 中 8μg TCS/g dw 也会影响沉积物细菌群落。然而，底栖大型无脊椎动物的存在并没有在未添加（即对照）沉积物中引起细菌群落的可测量变化。这些结果表明，TCS 单独在环境相关浓度（高达 8μg/g dw sed）下不会改变沉积物细菌群落，但可能与底栖大型无脊椎动物的存在一起产生影响。因此，我们建议在评估暴露于有机污染物等环境胁迫时，底栖大型无脊椎动物对沉积物细菌群落的响应时，将其纳入考量。
