Wang Long, Liu Jie, Yuan Jialin, Wang Nengfei
Department of Bioengineering, College of Marine Sciences and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
School of Chemistry and Chemical Engineering, Linyi University, Linyi 276012, China.
Microorganisms. 2022 Sep 27;10(10):1913. doi: 10.3390/microorganisms10101913.
Global climate change has caused the changes of the ecological environment in the Arctic region, including sea ice melting, runoff increase, glacial lake expansion, and a typical meltwater area has formed in the Arctic coastal area. In this study, the meltwater areas near six different characteristic areas of Ny-Ålesund in 2018 were taken as the research objects, and high-throughput sequencing of V3-V4 regions of all samples were performed using 16S rDNA. Among the soil samples of six glacial meltwater areas in Ny-Ålesund, Arctic, the meltwater area near the reservoir bay had the highest bacterial abundance, and the meltwater area near the sand had the lowest one. The dominant phyla in soil samples were Proteobacteria, Actinobacteria, Acidobacteria. The NH-N content in intertidal soil was higher than that in subtidal soil. Through WGCNA analysis and LEFSE analysis, it was found that the core bacteria significantly related to NH-N were basically distributed in the intertidal area. For example, , and were the core bacteria showed significant different abundance in the intertidal area, which have the ability to metabolize NH-N. Our findings suggest that NH-N plays an important role in soil bacterial community structure in the Arctic meltwater areas.
全球气候变化导致北极地区生态环境发生变化，包括海冰融化、径流增加、冰川湖扩张，北极沿海地区形成了典型的融水区域。本研究以2018年北极斯瓦尔巴群岛新奥尔松6个不同特征区域附近的融水区域为研究对象，采用16S rDNA对所有样本的V3-V4区进行高通量测序。在北极斯瓦尔巴群岛新奥尔松6个冰川融水区域的土壤样本中，水库湾附近的融水区域细菌丰度最高，沙地附近的融水区域细菌丰度最低。土壤样本中的优势菌门为变形菌门、放线菌门、酸杆菌门。潮间带土壤中NH-N含量高于潮下带土壤。通过WGCNA分析和LEFSE分析发现，与NH-N显著相关的核心细菌基本分布在潮间带区域。例如，、和是在潮间带区域表现出显著不同丰度的核心细菌，它们具有代谢NH-N的能力。我们的研究结果表明，NH-N在北极融水区域土壤细菌群落结构中起重要作用。
