Materials Genome Institute, Shanghai University, 200444 Shanghai, China.
Department of Chemistry, College of Science, Shanghai University, 200444 Shanghai, China.
Biosens Bioelectron. 2019 Apr 15;131:237-249. doi: 10.1016/j.bios.2019.02.019. Epub 2019 Feb 19.
In this mini review, we will briefly introduce the rapid development of DNA conformational polymorphism in biosensing field, including canonical DNA duplex, triplex, quadruplex, DNA origami, as well as more functionalized DNAs (aptamer, DNAzyme etc.). Various DNA structures are adopted to play important roles in sensor construction, through working as recognition receptor, signal reporter or linking staple for signal motifs, etc. We will mainly summarize their recent developments in DNA-based electrochemical and fluorescent sensors. For the electrochemical sensors, several types will be included, e.g. the amperometric, electrochemical impedance, electrochemiluminescence, as well as field-effect transistor sensors. For the fluorescent sensors, DNA is usually modified with fluorescent molecules or novel nanomaterials as report probes, excepting its core recognition function. Finally, general conclusion and future perspectives will be discussed for further developments.
在这篇迷你综述中，我们将简要介绍 DNA 构象多态性在生物传感领域的快速发展，包括典型的 DNA 双链体、三链体、四链体、DNA 折纸术以及更多功能化的 DNA（适体、DNA 酶等）。各种 DNA 结构被采用来在传感器构建中发挥重要作用，通过作为识别受体、信号报告器或连接信号基序的连接物等。我们将主要总结它们在基于 DNA 的电化学和荧光传感器中的最新发展。对于电化学传感器，将包括几种类型，例如电流测定法、电化学阻抗法、电致化学发光法以及场效应晶体管传感器。对于荧光传感器，DNA 通常被修饰为荧光分子或新型纳米材料作为报告探针，除了其核心识别功能。最后，将讨论一般结论和未来展望，以促进进一步发展。
