Aka Asuka, Matsuura Takashi, Yoshimura Atsutoshi
Department of Periodontology and Endodontology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki City 852-8588, Nagasaki, Japan.
J Funct Biomater. 2024 Aug 19;15(8):231. doi: 10.3390/jfb15080231.
The present study evaluated the cytocompatibility of three endodontic bioceramics in human periodontal-ligament-derived cells (hPDLCs): MTA Repair HP (HP), MTA Flow White (F), and Nishika Canal Sealer BG multi (BG). In addition, we also evaluated the effect of the powder-liquid (paste) ratio of F and BG on cytocompatibility. Discs of endodontic bioceramics (diameter = 8 mm, thickness = 1 mm) were prepared with HP, F, and BG. hPDLCs obtained from extracted teeth and cultured for three to five passages were used in the experiment. The prepared discs were placed at the bottom of a 48-well plate, seeded with hPDLCs at 100,000 cells/well, cultured for 7 or 28 days, and subjected to a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay. hPDLCs cultured without any discs were used as a negative control (NC) group. Discs made of F or BG mixed in three different consistencies were also used in this experiment. The absorbance values at days 7 and 28 were high in the order of HP > NC > BG > F. Furthermore, F or BG with higher consistency showed higher absorbance values. MTA Repair HP had the highest cytocompatibility among the three materials. Furthermore, it also showed that higher consistency improved cytocompatibility.
本研究评估了三种牙髓生物陶瓷材料在人牙周膜来源细胞（hPDLCs）中的细胞相容性：MTA Repair HP（HP）、MTA Flow White（F）和Nishika Canal Sealer BG multi（BG）。此外，我们还评估了F和BG的粉液（糊剂）比例对细胞相容性的影响。用HP、F和BG制备牙髓生物陶瓷圆盘（直径 = 8 mm，厚度 = 1 mm）。实验使用从拔除牙齿中获取并培养三至五代的hPDLCs。将制备好的圆盘置于48孔板底部，每孔接种100,000个hPDLCs，培养7天或28天，然后进行3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四氮唑测定。未放置任何圆盘培养的hPDLCs用作阴性对照（NC）组。本实验还使用了由F或BG以三种不同稠度混合制成的圆盘。在第7天和第28天的吸光度值按HP > NC > BG > F的顺序排列。此外，稠度较高的F或BG显示出较高的吸光度值。MTA Repair HP在这三种材料中具有最高的细胞相容性。此外，结果还表明较高的稠度可改善细胞相容性。
