枯草芽孢杆菌和金黄色葡萄球菌中的单链质粒DNA。
Single-stranded plasmid DNA in Bacillus subtilis and Staphylococcus aureus.
作者信息
te Riele H, Michel B, Ehrlich S D
出版信息
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2541-5. doi: 10.1073/pnas.83.8.2541.
Plasmid pC194 was found to exist in a double-stranded and a single-stranded DNA form in Bacillus subtilis and Staphylococcus aureus. This single-stranded DNA was found as a circular molecule of the same size as the parental monomer and corresponded to only one of the two DNA strands. It represented one-third of plasmid copies. Single- and double-stranded DNA copies in similar proportions to the above were detected for five other S. aureus plasmids (pC221, pC223, pE194, pT127, and pT181) and one B. subtilis plasmid (pHV416). S. aureus plasmid pUB110 and Bacillus cereus plasmid pBC16 were, in contrast, predominantly double-stranded.
人们发现质粒pC194在枯草芽孢杆菌和金黄色葡萄球菌中以双链和单链DNA形式存在。这种单链DNA是一种环状分子，其大小与亲本单体相同，且仅对应于两条DNA链中的一条。它占质粒拷贝数的三分之一。对于其他五种金黄色葡萄球菌质粒（pC221、pC223、pE194、pT127和pT181）和一种枯草芽孢杆菌质粒（pHV416），也检测到了比例与上述相似的单链和双链DNA拷贝。相比之下，金黄色葡萄球菌质粒pUB110和蜡样芽孢杆菌质粒pBC16主要是双链的。
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