Rudolph C F, Schmidt B J, Saunders C W
J Bacteriol. 1986 Mar;165(3):1015-8. doi: 10.1128/jb.165.3.1015-1018.1986.
The single-stranded form of a pE194-based plasmid transformed Bacillus subtilis protoplasts at least as efficiently as did the double-stranded plasmid, but the single-stranded form did not detectably transform B. subtilis competent cells.
基于pE194的质粒的单链形式转化枯草芽孢杆菌原生质体的效率至少与双链质粒一样高,但单链形式不能有效地转化枯草芽孢杆菌感受态细胞。