The John Curtin School of Medical Research, The Australian National University, Canberra, Australia.
ANU Medical School, The Australian National University, Canberra, Australia.
J Biophotonics. 2019 Jul;12(7):e201900010. doi: 10.1002/jbio.201900010. Epub 2019 Apr 1.
Red-light treatment is emerging as a novel therapy for promoting tissue recovery but data on red-light penetration through human tissues are lacking. We aimed to: (1) determine the effect of light irradiance, tissue thickness, skin tone, sex and bone/muscle content on 660 nm light penetration through common sites of sports injuries, and (2) establish if cadaver tissues serve as a useful model for predicting red-light penetration in live tissues. Live and cadaver human tissues were exposed to 660 nm light at locations across the skull, spinal cord and upper and lower limbs. Red-light was produced by a light emitting diode array of various irradiances (15-500 mW/cm ) and measured by a light-probe positioned on the tissue surface opposite to the light emitting diodes. 100 mW/cm successfully penetrated tissue <50 mm thick; a disproportionate irradiance increase was required to achieve deeper penetration. Penetration was unaffected by skin tone, increased with irradiance and relative bone/muscle composition, and decreased with greater tissue thickness and in males. Live and cadaveric tissue penetration did not differ statistically for tissues <50 mm but cadavers required more red-light to penetrate >50 mm. These results assist clinicians and researchers in determining red-light treatment intensities for penetrating human tissues.
红光治疗作为一种促进组织恢复的新疗法正在兴起，但缺乏关于红光穿透人体组织的数据。我们旨在：（1）确定光辐照度、组织厚度、肤色、性别和骨骼/肌肉含量对 660nm 光穿透常见运动损伤部位的影响；（2）确定尸体组织是否可作为预测活体组织中红光穿透的有用模型。将活人和尸体人体组织暴露于颅骨、脊髓和上下肢各处的 660nm 光下。发光二极管阵列产生的红光辐照度为 15-500mW/cm，通过位于与发光二极管相对的组织表面的光探头进行测量。100mW/cm 的光成功穿透厚度<50mm 的组织；要实现更深的穿透，需要不成比例的辐照度增加。穿透不受肤色影响，随辐照度和相对骨骼/肌肉组成增加，随组织厚度增加和男性而减少。对于<50mm 的组织，活体和尸体组织的穿透没有统计学差异，但尸体需要更多的红光才能穿透>50mm。这些结果有助于临床医生和研究人员确定穿透人体组织的红光治疗强度。
