爆轰纳米金刚石-BODIPY-酞菁纳米组装体的光物理性质和光动力治疗活性。
Photophysical properties and photodynamic therapy activities of detonated nanodiamonds-BODIPY-phthalocyanines nanoassemblies.
机构信息
Centre for Nanotechnology Innovation, Department of Chemistry, Rhodes University, South Africa.
Centre for Nanotechnology Innovation, Department of Chemistry, Rhodes University, South Africa.
出版信息
Photodiagnosis Photodyn Ther. 2019 Jun;26:101-110. doi: 10.1016/j.pdpdt.2019.03.007. Epub 2019 Mar 6.
This work reports on the synthesis of nanoassemblies of supramolecular hybrids containing detonated nanodiamonds (DNDs) covalently linked to halogenated BODIPY (DNDs-BODIPY) by an amide bond, followed by π-π stacking of 2,9,16,23-tetrakis[4-(N-methylpyridyloxy)]-phthalocyanine (ZnTPPcQ) on the DNDs-BODIPY conjugate, to form nanoassembly represented as ZnTPPcQ-DNDs-BODIPY. ZnTPPcQ-DNDs-BODIPY has a higher singlet oxygen quantum yield of 0.50 in water. Therefore, the construction of a three component photodynamic therapy agent (ZnTPPcQ-DNDs-BODIPY) as a single photosentisiser improved singlet quantum yields of the Pc. Zeta potential studies of ZnTPPcQ-DNDs-BODIPY under various temperatures, concentrations and pH conditions, showed the conjugate is more stable at pHs 2, 4 and 7 and at high concentrations (50 μg/mL) and temperatures (80 °C). ZnTPPcQ-DNDs-BODIPY showed high photodynamic therapy (PDT) activity with a low MCF-7 cell viability of 21 ± 5% when compared to 31 ± 2%, 30 ± 2% and 28 ± 2% cell viability at the highest tested concentration of 50 μg/mL for DNDs, ZnTPPcQ-DND and DNDs-BODIPY, respectively.
这项工作报道了超分子杂化物纳米组装体的合成，该杂化物包含通过酰胺键共价连接的爆炸纳米金刚石（DND）和卤化 BODIPY（DND-BODIPY），随后 2,9,16,23-四[4-（N-甲基吡啶氧基）]酞菁锌（ZnTPPcQ）通过π-π堆积在 DND-BODIPY 缀合物上，形成代表为 ZnTPPcQ-DNDs-BODIPY 的纳米组装体。ZnTPPcQ-DNDs-BODIPY 在水中具有更高的单线态氧量子产率为 0.50。因此，构建三组分光动力治疗剂（ZnTPPcQ-DNDs-BODIPY）作为单一光敏剂可提高卟啉的单线态量子产率。在各种温度、浓度和 pH 值条件下对 ZnTPPcQ-DNDs-BODIPY 的zeta 电位研究表明，该缀合物在 pH 值为 2、4 和 7 以及高浓度（50μg/mL）和温度（80°C）下更稳定。与 DNDs、ZnTPPcQ-DND 和 DNDs-BODIPY 的最高测试浓度 50μg/mL 相比，ZnTPPcQ-DNDs-BODIPY 具有较高的光动力治疗（PDT）活性，MCF-7 细胞活力仅为 21±5%。
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