Centre for Nanotechnology Innovation, Department of Chemistry, Rhodes University, South Africa.
Centre for Nanotechnology Innovation, Department of Chemistry, Rhodes University, South Africa.
Photodiagnosis Photodyn Ther. 2019 Jun;26:101-110. doi: 10.1016/j.pdpdt.2019.03.007. Epub 2019 Mar 6.
This work reports on the synthesis of nanoassemblies of supramolecular hybrids containing detonated nanodiamonds (DNDs) covalently linked to halogenated BODIPY (DNDs-BODIPY) by an amide bond, followed by π-π stacking of 2,9,16,23-tetrakis[4-(N-methylpyridyloxy)]-phthalocyanine (ZnTPPcQ) on the DNDs-BODIPY conjugate, to form nanoassembly represented as ZnTPPcQ-DNDs-BODIPY. ZnTPPcQ-DNDs-BODIPY has a higher singlet oxygen quantum yield of 0.50 in water. Therefore, the construction of a three component photodynamic therapy agent (ZnTPPcQ-DNDs-BODIPY) as a single photosentisiser improved singlet quantum yields of the Pc. Zeta potential studies of ZnTPPcQ-DNDs-BODIPY under various temperatures, concentrations and pH conditions, showed the conjugate is more stable at pHs 2, 4 and 7 and at high concentrations (50 μg/mL) and temperatures (80 °C). ZnTPPcQ-DNDs-BODIPY showed high photodynamic therapy (PDT) activity with a low MCF-7 cell viability of 21 ± 5% when compared to 31 ± 2%, 30 ± 2% and 28 ± 2% cell viability at the highest tested concentration of 50 μg/mL for DNDs, ZnTPPcQ-DND and DNDs-BODIPY, respectively.
这项工作报道了超分子杂化物纳米组装体的合成，该杂化物包含通过酰胺键共价连接的爆炸纳米金刚石（DND）和卤化 BODIPY（DND-BODIPY），随后 2,9,16,23-四[4-（N-甲基吡啶氧基）]酞菁锌（ZnTPPcQ）通过π-π堆积在 DND-BODIPY 缀合物上，形成代表为 ZnTPPcQ-DNDs-BODIPY 的纳米组装体。ZnTPPcQ-DNDs-BODIPY 在水中具有更高的单线态氧量子产率为 0.50。因此，构建三组分光动力治疗剂（ZnTPPcQ-DNDs-BODIPY）作为单一光敏剂可提高卟啉的单线态量子产率。在各种温度、浓度和 pH 值条件下对 ZnTPPcQ-DNDs-BODIPY 的zeta 电位研究表明，该缀合物在 pH 值为 2、4 和 7 以及高浓度（50μg/mL）和温度（80°C）下更稳定。与 DNDs、ZnTPPcQ-DND 和 DNDs-BODIPY 的最高测试浓度 50μg/mL 相比，ZnTPPcQ-DNDs-BODIPY 具有较高的光动力治疗（PDT）活性，MCF-7 细胞活力仅为 21±5%。
