Bondon Nicolas, Durand Denis, Hadj-Kaddour Kamel, Ali Lamiaa M A, Boukherroub Rabah, Bettache Nadir, Gary-Bobo Magali, Raehm Laurence, Durand Jean-Olivier, Nguyen Christophe, Charnay Clarence
ICGM, University of Montpellier, UMR-CNRS 5253, 34293 Montpellier, France.
IBMM, University of Montpellier, UMR-CNRS 5247, 34293 Montpellier, France.
Life (Basel). 2022 Dec 7;12(12):2044. doi: 10.3390/life12122044.
In addition to their great optical properties, nanodiamonds (NDs) have recently proved useful for two-photon-excited photodynamic therapy (TPE-PDT) applications. Indeed, they are able to produce reactive oxygen species (ROS) directly upon two-photon excitation but not with one-photon excitation; Methods: Fluorescent NDs (FNDs) with a 100 nm diameter and detonation NDs (DNDs) of 30 nm were compared. In order to use the gems for cancer-cell theranostics, they were encapsulated in a bis(triethoxysilyl)ethylene-based (ENE) periodic mesoporous organosilica (PMO) shell, and the surface of the formed nanoparticles (NPs) was modified by the direct grafting of polyethylene glycol (PEG) and amino groups using PEG-hexyltriethoxysilane and aminoundecyltriethoxysilane during the sol-gel process. The NPs' phototoxicity and interaction with MDA-MB-231 breast cancer cells were evaluated afterwards; Results: Transmission electronic microscopy images showed the formation of core-shell NPs. Infrared spectra and zeta-potential measurements confirmed the grafting of PEG and NH groups. The encapsulation of the NDs allowed for the imaging of cancer cells with NDs and for the performance of TPE-PDT of MDA-MB-231 cancer cells with significant mortality.
Multifunctional ND@PMO core-shell nanosystems were successfully prepared. The NPs demonstrated high biocompatibility and TPE-PDT efficiency in vitro in the cancer cell model. Such systems hold good potential for two-photon-excited PDT applications.
除了具有出色的光学特性外，纳米金刚石（NDs）最近已被证明可用于双光子激发光动力疗法（TPE-PDT）应用。实际上，它们能够在双光子激发时直接产生活性氧（ROS），但单光子激发时则不能。
比较了直径为100nm的荧光纳米金刚石（FNDs）和30nm的爆轰纳米金刚石（DNDs）。为了将这些纳米颗粒用于癌细胞的诊疗，将它们封装在基于双（三乙氧基硅基）乙烯（ENE）的周期性介孔有机硅（PMO）壳中，并在溶胶-凝胶过程中使用聚乙二醇己基三乙氧基硅烷和氨基十一烷基三乙氧基硅烷通过直接接枝聚乙二醇（PEG）和氨基对形成的纳米颗粒（NPs）表面进行修饰。随后评估了这些纳米颗粒的光毒性以及它们与MDA-MB-231乳腺癌细胞的相互作用。
透射电子显微镜图像显示了核壳纳米颗粒的形成。红外光谱和ζ电位测量证实了PEG和NH基团的接枝。纳米金刚石的封装使得能够用纳米金刚石对癌细胞进行成像，并对MDA-MB-231癌细胞进行TPE-PDT，具有显著的死亡率。
成功制备了多功能ND@PMO核壳纳米系统。这些纳米颗粒在癌细胞模型中体外表现出高生物相容性和TPE-PDT效率。此类系统在双光子激发光动力疗法应用中具有良好的潜力。
