G.A. Krestov Institute of Solution Chemistry of Russian Academy of Sciences, 1 Akademicheskaya St., 153045 Ivanovo, Russia.
Inorganic Chemistry Department, Ivanovo State University of Chemistry and Technology, 7 Sheremetevskiy Ave., 153000 Ivanovo, Russia.
Molecules. 2022 Feb 18;27(4):1396. doi: 10.3390/molecules27041396.
Fluorescent dyes absorbing and emitting in the visible and near-IR regions are promising for the development of fluorescent probes for labeling and bio-visualization of body cells. The ability to absorb and emit in the long-wavelength region increases the efficiency of recording the spectral signals of the probes due to the higher permeability of the skin layers. Compared to other fluorescent dyes, BODIPYs are attractive due to their excellent photophysical properties-narrow absorption and emission, intense fluorescence, simple signal modulation for the practical applications. As part of conjugates with biomolecules, BODIPY could act as a biomarker, but as therapeutic agent, which allows solving several problems at once-labeling or bioimaging and treatment based on the suppression of pathogenic microflora and cancer cells, which provides a huge potential for practical application of BODIPY conjugates in medicine. The review is devoted to the discussion of the recent, promising directions of BODIPY application in the field of conjugation with biomolecules. The first direction is associated with the development of BODIPY conjugates with drugs, including compounds of platinum, paclitaxel, chlorambucil, isoxazole, capsaicin, etc. The second direction is devoted to the labeling of vitamins, hormones, lipids, and other biomolecules to control the processes of their transport, localization in target cells, and metabolism. Within the framework of the third direction, the problem of obtaining functional optically active materials by conjugating BODIPY with other colored and fluorescent particles, in particular, phthalocyanines, is being solved.
在可见和近红外区域吸收和发射荧光染料对于开发用于标记和生物可视化体细胞的荧光探针具有广阔的前景。由于皮肤层的通透性更高，在长波长区域吸收和发射的能力提高了探针光谱信号记录的效率。与其他荧光染料相比，BODIPY 因其出色的光物理性质（窄吸收和发射、强荧光、简单的信号调制）而具有吸引力，这些性质使其在实际应用中具有吸引力。作为与生物分子缀合物的一部分，BODIPY 可以作为生物标志物，但也可以作为治疗剂，这可以同时解决几个问题——基于抑制病原微生物和癌细胞的标记或生物成像和治疗，这为 BODIPY 缀合物在医学中的实际应用提供了巨大的潜力。该综述致力于讨论 BODIPY 在与生物分子缀合领域中的最新有前途的应用方向。第一个方向与开发与药物的 BODIPY 缀合物有关，包括铂、紫杉醇、氯苯丁酸、异恶唑、辣椒素等化合物。第二个方向致力于标记维生素、激素、脂质和其他生物分子，以控制它们的运输、在靶细胞中的定位和代谢过程。在第三个方向的框架内，通过将 BODIPY 与其他有色和荧光颗粒（特别是酞菁）缀合来获得功能性光学活性材料的问题正在得到解决。
