Department of Medical Oncology, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou 221009, China.
Department of Molecular Laboratory, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou 221009, China.
Biomed Pharmacother. 2019 May;113:108686. doi: 10.1016/j.biopha.2019.108686. Epub 2019 Mar 6.
Long non-coding RNA small ubiquitin-like modifier 1 pseudogene 3 (SUMO1P3) is located on chromosome 1q23.2, and has been suggested to serve as oncogenic lncRNA in many kinds of human malignancy. The role of SUMO1P3 in non-small cell lung cancer (NSCLC) was still unknown. In our study, we analyzed The Cancer Genome Atlas (TCGA) database, and observed SUMO1P3 expression was increased in both lung squamous cell carcinoma and lung adenocarcinoma. Then, we confirmed that SUMO1P3 expression was significantly increased in NSCLC cancer tissues and cell lines. Meanwhile, the expression levels of SUMO1P3 expression in metastatic lymph node specimens were strikingly elevated in comparison to primary NSCLC tissue specimens. Then, we found high SUMO1P3 expression was correlated with late clinical stage, lymph node metastasis, distant metastasis and poor differentiated degree. In the survival analysis of TCGA, we observed that SUMO1P3 expression had no association with overall survival and disease free survival in NSCLC patients. There was a statistically negative correlation between SUMO1P3 expression and miR-136 expression in NSCLC tissues. Moreover, miR-136 directly bound to SUMO1P3, and SUMO1P3 negatively regulated miR-136 expression in NSCLC cells. Furthermore, SUMO1P3 promoted NSCLC cell migration and invasion via regulating miR-136. In conclusion, SUMO1P3 functions as metastasis-associated lncRNA in NSCLC.
长链非编码 RNA 小泛素样修饰物 1 假基因 3（SUMO1P3）位于 1q23.2 染色体上，在许多人类恶性肿瘤中被认为是致癌的 lncRNA。SUMO1P3 在非小细胞肺癌（NSCLC）中的作用尚不清楚。在我们的研究中，我们分析了癌症基因组图谱（TCGA）数据库，观察到 SUMO1P3 在肺鳞癌和肺腺癌中均表达增加。然后，我们证实 SUMO1P3 在 NSCLC 癌组织和细胞系中表达显著增加。同时，与原发性 NSCLC 组织标本相比，转移性淋巴结标本中 SUMO1P3 表达水平明显升高。然后，我们发现高 SUMO1P3 表达与晚期临床分期、淋巴结转移、远处转移和低分化程度相关。在 TCGA 的生存分析中，我们观察到 SUMO1P3 表达与 NSCLC 患者的总生存和无病生存无关。在 NSCLC 组织中，SUMO1P3 表达与 miR-136 表达呈统计学负相关。此外，miR-136 直接与 SUMO1P3 结合，SUMO1P3 在 NSCLC 细胞中负调控 miR-136 的表达。此外，SUMO1P3 通过调节 miR-136 促进 NSCLC 细胞迁移和侵袭。总之，SUMO1P3 在 NSCLC 中作为转移相关的 lncRNA 发挥作用。
