Medical College, Henan University of Science and Technology, Luoyang 471003, Henan, China.
Aging (Albany NY). 2021 Apr 26;13(9):12479-12492. doi: 10.18632/aging.202921.
Long noncoding RNAs (lncRNAs) are involved in the progression of various cancers, including hepatocellular carcinoma (HCC). However, the biological functions of lncRNA small ubiquitin-like modifier 1 pseudogene 3 (SUMO1P3) and the underlying mechanisms remain unclear. In this study, we revealed that SUMO1P3 expression was enhanced in HCC tissues and cell lines, positively associating with tumor size and number, poor differentiation, lymphatic and distant metastasis, TNM stage, and poor prognosis in HCC patients. assays showed that SUMO1P3 depletion reduced HCC cell viability and proliferation by hindering cyclin D1 expression and Akt phosphorylation. SUMO1P3 knockdown induced HCC cell apoptosis, as indicated by increased Bax and cleaved caspase-3 expression and the decreased Bcl-2 level. SUMO1P3 silencing suppressed HCC cell migration and invasion by increasing epithelial marker E-cadherin expression and decreasing mesenchymal marker vimentin expression, as well as reducing matrix metalloproteinase (MMP)-2 and MMP-9 levels. Consistently, SUMO1P3 depletion in HCC cells retarded tumor growth and lung metastasis . Overall, these results supported the applicability of SUMO1P3 as a useful predictor of HCC prognosis and a potential therapeutic target for HCC patients.
长链非编码 RNA（lncRNA）参与多种癌症的进展，包括肝细胞癌（HCC）。然而，lncRNA 小泛素样修饰物 1 假基因 3（SUMO1P3）的生物学功能及其潜在机制尚不清楚。在本研究中，我们揭示了 SUMO1P3 在 HCC 组织和细胞系中表达增强，与肿瘤大小和数量、分化不良、淋巴和远处转移、TNM 分期以及 HCC 患者的预后不良呈正相关。功能测定表明，SUMO1P3 耗竭通过抑制细胞周期蛋白 D1 表达和 Akt 磷酸化来降低 HCC 细胞活力和增殖。SUMO1P3 敲低诱导 HCC 细胞凋亡，表现为 Bax 和 cleaved caspase-3 表达增加和 Bcl-2 水平降低。SUMO1P3 沉默通过增加上皮标志物 E-钙黏蛋白表达和降低间充质标志物波形蛋白表达，以及降低基质金属蛋白酶（MMP）-2 和 MMP-9 水平，抑制 HCC 细胞迁移和侵袭。一致地，HCC 细胞中 SUMO1P3 的耗竭可减缓肿瘤生长和肺转移。总的来说，这些结果支持 SUMO1P3 作为 HCC 预后的有用预测因子和 HCC 患者潜在治疗靶点的适用性。
