Mühlpfordt H, Schottelius J
Tropenmed Parasitol. 1977 Mar;28(1):1-7.
Protectin from the sponge Aaptos papillata (Keller) was used in the characterization of five strains of T. cruzi (Venezuela, Guatemala, Y. Brasilien, Peru, Wien) and six T. cruzi like strains (Triatoma, Maryland, ITMAP 943, FH4, FH5, LN). Based upon their membrane receptors, these T. cruzi and T. cruzi like isolates could be differentiated from rangeli (Venezuela Strain) and T. conorhini (Hawai Strain) by agglutination reaction to the proctectin. Furthermore, after pronase treatment T.rangeli could also be distinguished from T. conorhini by agglutination test with A. papillata protectin and also Soja hispida lectin. It is not possible to differentiate the T. cruzi complex with S. hispida lectin, because it did not agglutinate T. cruzi (Vienna Strain) and T. cruzi like (Maryland Strain). However, after treating this human pathogenic strain with pronase the pseudocrypt antigen of the first order is made available to the S. hispida lecting thereby producing agglutination. The T. cruzi like strain however did not agglutinate with this treatment. On the other hand, while T. rangeli did not agglutinate even after pronase treatment, T. conorhini showed the agglutination reaction. This observed reaction is explained by the availability of the pseudocrypt antigens of the first order after pronase treatment.
来自海绵Aaptos papillata（凯勒）的保护素被用于对五株克氏锥虫（委内瑞拉、危地马拉、巴西Y、秘鲁、维也纳）和六株类克氏锥虫菌株（锥蝽、马里兰、ITMAP 943、FH4、FH5、LN）进行特性分析。基于它们的膜受体，这些克氏锥虫和类克氏锥虫分离株通过对保护素的凝集反应可与兰氏锥虫（委内瑞拉菌株）和康氏锥虫（夏威夷菌株）区分开来。此外，在蛋白酶处理后，兰氏锥虫也可通过与A. papillata保护素以及大豆凝集素的凝集试验与康氏锥虫区分开来。用大豆凝集素无法区分克氏锥虫复合体，因为它不会凝集克氏锥虫（维也纳菌株）和类克氏锥虫（马里兰菌株）。然而，在用蛋白酶处理这种人类致病菌株后，一级假隐抗原可被大豆凝集素利用，从而产生凝集反应。而类克氏锥虫菌株经此处理后不会凝集。另一方面，虽然兰氏锥虫即使在蛋白酶处理后也不会凝集，但康氏锥虫会出现凝集反应。观察到的这种反应可通过蛋白酶处理后一级假隐抗原的可利用性来解释。
