Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 510632, China.
Guangdong Key Laboratory of Integrated Agro-Environmental Pollution Control and Management, Guangdong Institute of Eco-Environmental Science and Technology, Guangzhou, 510650, China.
Antonie Van Leeuwenhoek. 2019 Aug;112(8):1213-1219. doi: 10.1007/s10482-019-01253-z. Epub 2019 Mar 9.
A Gram-staining negative bacterium, designated as GSS15, was isolated from compost in Guangzhou, China. Cells of strain GSS15 were rod-shaped and non-motile. The isolate was able to grow at 15-42 °C (optimum 30 °C) and pH 6.0-11.0 (optimum pH 8.0), and tolerate up to 6.0% NaCl (w/v). When the 16S rRNA gene sequence of the isolate was compared with those of other bacteria, the highest similarity was observed with Pseudoxanthomonas helianthi roo10 (96.9%). Furthermore, strain GSS15 showed low ANI (75.7-79.5%) and DDH (24.2-18.3%) values to the closely related species. Q-8 was the predominant respiratory quinone. The major cellular fatty acids ( > 5%) were iso-C (18.7%), Cω7c (18.6%), anteiso-C (13.2%), C (9.8%), and iso-C (8.8%). The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. Based on its phenotypic, chemotaxonomic and genotypic data, strain GSS15 (= KCTC 52974 = MCCC 1K03334) is designated as the type strain of a novel species of the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas composti sp. nov. is proposed.
一株革兰氏染色阴性细菌，命名为 GSS15，从中国广州的堆肥中分离得到。GSS15 菌株的细胞呈杆状，不运动。该分离物能够在 15-42°C（最佳 30°C）和 pH 6.0-11.0（最佳 pH 8.0）下生长，并耐受高达 6.0%（w/v）的 NaCl。当比较该分离物的 16S rRNA 基因序列与其他细菌的序列时，与 Pseudoxanthomonas helianthi roo10（96.9%）的相似度最高。此外，GSS15 菌株与亲缘关系较近的物种的 ANI（75.7-79.5%）和 DDH（24.2-18.3%）值较低。Q-8 是主要的呼吸醌。主要的细胞脂肪酸（ > 5%）为 iso-C（18.7%）、Cω7c（18.6%）、anteiso-C（13.2%）、C（9.8%）和 iso-C（8.8%）。极性脂类由磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油组成。基于其表型、化学分类和基因型数据，GSS15 菌株（= KCTC 52974 = MCCC 1K03334）被指定为 Pseudoxanthomonas 属的一个新种的模式株，该新种被命名为 Pseudoxanthomonas composti sp. nov.。
