Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, People's Republic of China.
Antonie Van Leeuwenhoek. 2013 Sep;104(3):369-76. doi: 10.1007/s10482-013-9959-4. Epub 2013 Jul 18.
A thermotolerant Gram-staining negative and aerobic bacterium, designated strain YIM 77520(T), was isolated from a geothermally heated soil sample collected at Rehai National Park, Tengchong, Yunnan Province, South-West China. Cells of the strain were found to be rod-shaped and colonies were light beige and circular. The strain was found to grow in the presence of 0-2 % (w/v) total salts (optimum, 0 %), at pH 6.0-8.0 (optimum, pH 7.0) and 25-55 °C (optimum, 45 °C). The only quinone detected was Q-8 and the genomic DNA G+C content was determined to be 66.9 mol%. The major fatty acids (>10 %) were identified as iso-C16:0 and iso-C15:0. The phospholipids were found to consist of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, five unknown phospholipids and three aminophospholipids. Based on the 16S rRNA gene sequence analysis, strain YIM 77520(T) was found to form a cluster with Lysobacter thermophilus YIM 77875(T) and showed the highest 16S rRNA gene sequence similarity to L. thermophilus YIM 77875(T) (96.0 %). These two strains formed a distinct lineage of the family 'Xanthomonadaceae'. On the basis of the morphological and chemotaxonomic characteristics, as well as genotypic data, a new genus, Vulcaniibacterium gen. nov. is proposed with Vulcaniibacterium tengchongense sp. nov. as the type species. The type strain of V. tengchongense sp. nov. is strain YIM 77520(T) (=DSM 25623(T) = CCTCC AB 2011152(T)). Furthermore we propose that L. thermophilus Wei et al. 2012 is reclassified in the new genus as Vulcaniibacterium thermophilum comb. nov. (type strain YIM 77875(T) = CCTCC AB 2012064(T) = KCTC 32020(T)) based on polyphasic data.
一株耐热革兰氏阴性需氧菌，命名为 YIM 77520(T) 菌株，从中国西南部云南省腾冲热海国家公园采集的地热土壤样本中分离得到。该菌株的细胞呈杆状，菌落呈浅米色圆形。该菌株在 0-2%（w/v）总盐（最佳，0%）、pH 值 6.0-8.0（最佳，pH 值 7.0）和 25-55°C（最佳，45°C）的条件下生长。检测到的唯一醌类是 Q-8，基因组 DNA G+C 含量为 66.9mol%。主要脂肪酸（>10%）为 iso-C16:0 和 iso-C15:0。磷脂由磷脂乙醇胺、磷脂甘油、双磷脂酰甘油、五种未知磷脂和三种氨基磷脂组成。根据 16S rRNA 基因序列分析，YIM 77520(T) 菌株与 Lysobacter thermophilus YIM 77875(T) 形成一个聚类，与 L. thermophilus YIM 77875(T) 的 16S rRNA 基因序列相似度最高（96.0%）。这两个菌株形成了一个独特的黄单胞菌科家族谱系。基于形态学和化学分类特征以及基因型数据，提出了一个新属，Vulcaniibacterium 属，新种 Vulcaniibacterium tengchongense sp. nov. 作为模式种。V. tengchongense sp. nov. 的模式菌株为 YIM 77520(T)=DSM 25623(T)=CCTCC AB 2011152(T)。此外，我们建议将 Wei 等人 2012 年提出的 L. thermophilus 重新分类到新属 Vulcaniibacterium thermophilum comb. nov. 中，其模式菌株为 YIM 77875(T)=CCTCC AB 2012064(T)=KCTC 32020(T)。
