Hao Ming-Hua, Zhang Fan, Liu Xing-Xia, Zhang Fan, Wang Li-Juan, Xu Sai-Juan, Zhang Jin-Hua, Ji Hong-Long, Xu Ping
Department of Pharmacy, Xinxiang Medical University, Xinxiang, Henan, 453003, China.
The Third Affiliated Hospital, Xinxiang Medical University, Xinxiang, Henan, 453003, China.
Trop J Pharm Res. 2018;17(1):71-76. doi: 10.4314/tjpr.v17i1.11.
To perform a qualitative and quantitative analysis of catechin and quercetin in flavonoids extracted from Rosa roxburghii Tratt.
Total flavonoids were determined using ultraviolet spectrophotometry (UV) at 500 nm. The optimal gradient program started with 15 % methanol and was kept within a period of 0 - 20 min, while 25 % methanol was kept within 20 - 33 min. Subsequently, the concentration of methanol was reduced to 15 % and was held for 10 min until the next injection. Mass spectrometry spray voltage was 4,000 V, ionization temperature 350 °C, atomizer pressure 35 psi, nitrogen flow rate 8 L/min, and mass scan range 200 - 800 m/z. The detection wavelength used for catechin and quercetin was 270 and 368 nm, respectively.
Based on the UV results, Rosa roxburghii Tratt content was 73.85 %, which is in agreement with the national standard. Liquid chromatography-mass spectrometry (LC-MS) results indicate that Rosa roxburghii Tratt flavonoids contained quercetin, 34.26 %, with relative standard deviation (RSD) of 2.88 % and catechin content of 2.97 % with RSD of 1.49 %.
The proposed measurement method for determining the content of flavonoids in Rosa roxburghii Tratt has the advantage of simplicity, feasibility, good repeatability, and rapid and accurate analysis.
对刺梨中提取的黄酮类化合物中的儿茶素和槲皮素进行定性和定量分析。
采用紫外分光光度法（UV）在500nm波长处测定总黄酮含量。最佳梯度程序起始为15%甲醇，在0 - 20分钟内保持该浓度，20 - 33分钟内保持25%甲醇浓度。随后，甲醇浓度降至15%并保持10分钟直至下一次进样。质谱喷雾电压为4000V，离子化温度350℃，雾化器压力35psi，氮气流量8L/min，质量扫描范围200 - 800m/z。用于儿茶素和槲皮素的检测波长分别为270nm和368nm。
基于紫外结果，刺梨含量为73.85%，符合国家标准。液相色谱 - 质谱联用（LC - MS）结果表明，刺梨黄酮类化合物中槲皮素含量为34.26%，相对标准偏差（RSD）为2.88%，儿茶素含量为2.97%，RSD为1.49%。
所提出的测定刺梨中黄酮类化合物含量的方法具有操作简单、可行、重复性好以及分析快速准确的优点。
