Ping Xu, Junqing Jia, Junfeng Jia, Enjin Jiang
Department of Pharmacy, Xinxiang Medical University, Xinxiang, Henan, China.
Asian Pac J Cancer Prev. 2011;12(10):2593-6.
The purpose of this study was to determine radioprotective effects of troxerutin. Cell experiments were carried out to test the cytotoxicity of troxerutin on V79 cells and to observe effects on apoptosis caused by 60CO γ rays. A model of 8 Gy ray-caused damage of mice was established to observe the effect that troxerutin has on the physical symptom of irradiated mice and to calculate the 30-day survival rate. It showed that troxerutin had no obvious cytotoxicity at the level of less than 20 μg/ml; but had a redioprotective effect in dose-dependence on viability of V79 cells at the range of 0.2-5 μg/mL irradiated by 5 Gy ray of 60CO γ ray. After the 8 Gy irradiation, the mice lost some weight, were dried up in fur and feather, low spirit, awkward in movement, shrinking in body and handicapped in sight, while mice with troxerutin were much better. So it was clear that troxerutin could increase the 30-day survival rates of irradiated mice dramatically. These results collectively indicate that troxerutin is an effective radioprotective agent.
本研究的目的是确定曲克芦丁的辐射防护作用。进行细胞实验以测试曲克芦丁对V79细胞的细胞毒性，并观察其对60Coγ射线引起的细胞凋亡的影响。建立8 Gy射线致小鼠损伤模型，观察曲克芦丁对受照射小鼠身体症状的影响，并计算30天存活率。结果表明，曲克芦丁在浓度低于20μg/ml时无明显细胞毒性；但在0.2 - 5μg/mL范围内，对5 Gy的60Coγ射线照射的V79细胞活力具有剂量依赖性的辐射防护作用。8 Gy照射后，小鼠体重减轻，皮毛干枯，精神萎靡，行动不便，身体蜷缩，视力障碍，而给予曲克芦丁的小鼠情况要好得多。因此，曲克芦丁可显著提高受照射小鼠的30天存活率。这些结果共同表明曲克芦丁是一种有效的辐射防护剂。
