Klotz Peter, Higgins Paul G, Schaubmar Andreas R, Failing Klaus, Leidner Ursula, Seifert Harald, Scheufen Sandra, Semmler Torsten, Ewers Christa
Institute of Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, Germany.
Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany.
Front Microbiol. 2019 Feb 22;10:272. doi: 10.3389/fmicb.2019.00272. eCollection 2019.
is one of the leading causes of nosocomial infections in humans. To investigate its prevalence, distribution of sequence types (STs), and antimicrobial resistance in cattle, we sampled 422 cattle, including 280 dairy cows, 59 beef cattle, and 83 calves over a 14-month period. Metadata, such as the previous use of antimicrobial agents and feeding, were collected to identify putative determining factors. Bacterial isolates were identified via MALDI-TOF/MS and PCR, antimicrobial susceptibility was evaluated via VITEK2 and antibiotic gradient tests, resistance genes were identified by PCR. Overall, 15.6% of the cattle harbored , predominantly in the nose (60.3% of the isolates). It was more frequent in dairy cows (21.1%) than in beef cattle (6.8%) and calves (2.4%). A seasonal occurrence was shown with a peak between May and August. The rate of occurrence of was correlated with a history of use of 3rd generation cephalosporins in the last 6 months prior to sampling Multilocus sequence typing (Pasteur scheme) revealed 83 STs among 126 unique isolates. Nine of the bovine STs have previously been implicated in human infections. Besides known intrinsic resistance of the species, the isolates did not show additional resistance to the antimicrobial substances tested, including carbapenems. Our data suggest that cattle are not a reservoir for nosocomial but carry a highly diverse population of this species. Nevertheless, some STs seem to be able to colonize both cattle and humans.
是人类医院感染的主要原因之一。为了调查其在牛群中的流行情况、序列类型（STs）分布及抗菌药物耐药性，我们在14个月的时间里对422头牛进行了采样，其中包括280头奶牛、59头肉牛和83头犊牛。收集了诸如先前抗菌药物使用情况和饲养等元数据，以确定可能的决定因素。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF/MS）和聚合酶链反应（PCR）鉴定细菌分离株，通过VITEK2和抗生素梯度试验评估抗菌药物敏感性，通过PCR鉴定耐药基因。总体而言，15.6%的牛携带 ，主要存在于鼻腔（分离株的60.3%）。在奶牛（21.1%）中比在肉牛（6.8%）和犊牛（2.4%）中更常见。呈现出季节性发生，在5月至8月间达到高峰。 的发生率与采样前最后6个月内使用第三代头孢菌素的历史相关。多位点序列分型（巴斯德方案）在126个独特分离株中揭示了83种STs。其中9种牛的STs先前已涉及人类感染。除了该物种已知的固有耐药性外，分离株对所测试的抗菌物质（包括碳青霉烯类）未表现出额外耐药性。我们的数据表明，牛不是医院 的储存宿主，但携带该物种的高度多样化群体。然而，一些STs似乎能够在牛和人类中定殖。
