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乙烯信号通路对大豆中CBF/DREB调控的冷响应产生负面影响（）。

The Ethylene Signaling Pathway Negatively Impacts CBF/DREB-Regulated Cold Response in Soybean ().

作者信息

Robison Jennifer D, Yamasaki Yuji, Randall Stephen K

机构信息

Department of Biology, Indiana University-Purdue University Indianapolis, Indianapolis, IN, United States.

出版信息

Front Plant Sci. 2019 Feb 12;10:121. doi: 10.3389/fpls.2019.00121. eCollection 2019.

DOI:10.3389/fpls.2019.00121
PMID:30853961
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6396728/
Abstract

During cold stress, soybean CBF/DREB1 transcript levels increase rapidly; however, expected downstream targets appear unresponsive. Here, we asked whether the ethylene signaling pathway, which is enhanced in the cold can negatively regulate the soybean CBF/DREB1 cold responsive pathway; thus contributing to the relatively poor cold tolerance of soybean. Inhibition of the ethylene signaling pathway resulted in a significant increase in and transcripts, while stimulation led to decreased and transcripts. A cold responsive reporter construct (), as well as predicted downstream targets of soybean CBF/DREB1 [ (ADH), (ubiquitin ligase), (AP2), and (CYP)] were impacted by the modulation of the ethylene signaling pathway. Photosynthetic parameters were affected by ethylene pathway stimulation, but only at control temperatures. Freezing tolerance (as measured by electrolyte leakage), free proline, and MDA; in both acclimated and non-acclimated plants were increased by silver nitrate but not by other ethylene pathway inhibitors. This work provides evidence that the ethylene signaling pathway, possibly through the action of EIN3, transcriptionally inhibits the CBF/DREB1 pathway in soybean.

摘要

在冷胁迫期间，大豆CBF/DREB1转录水平迅速增加；然而，预期的下游靶标似乎没有反应。在此，我们探究了在冷胁迫下增强的乙烯信号通路是否会负向调节大豆CBF/DREB1冷响应通路；从而导致大豆相对较差的耐寒性。抑制乙烯信号通路导致 和 转录本显著增加，而刺激则导致 和 转录本减少。一个冷响应报告基因构建体（）以及大豆CBF/DREB1的预测下游靶标[ （ADH）、 （泛素连接酶）、 （AP2）和 （CYP）]受到乙烯信号通路调节的影响。光合参数受到乙烯通路刺激的影响，但仅在对照温度下。耐寒性（通过电解质渗漏测量）、游离脯氨酸和丙二醛；在驯化和未驯化的植物中，硝酸银均能提高其含量，但其他乙烯通路抑制剂则不能。这项工作提供了证据，表明乙烯信号通路可能通过EIN3的作用，在转录水平上抑制大豆中的CBF/DREB1通路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/a4beab3705ae/fpls-10-00121-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/fe2ea9be3798/fpls-10-00121-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/4d2c9616a880/fpls-10-00121-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/99dae0e1e75b/fpls-10-00121-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/732f60ec365c/fpls-10-00121-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/a74645c47f01/fpls-10-00121-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/52f22ca906cc/fpls-10-00121-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/ddf9dd718022/fpls-10-00121-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/7b78c0e399cf/fpls-10-00121-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/a4beab3705ae/fpls-10-00121-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/fe2ea9be3798/fpls-10-00121-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/4d2c9616a880/fpls-10-00121-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/99dae0e1e75b/fpls-10-00121-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/732f60ec365c/fpls-10-00121-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/a74645c47f01/fpls-10-00121-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/52f22ca906cc/fpls-10-00121-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/ddf9dd718022/fpls-10-00121-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/7b78c0e399cf/fpls-10-00121-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a87/6396728/a4beab3705ae/fpls-10-00121-g009.jpg

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