Jinan Stomatological Hospital, Jinan, China.
J Cell Biochem. 2019 Aug;120(8):12429-12435. doi: 10.1002/jcb.28509. Epub 2019 Mar 10.
Tongue squamous cell carcinoma (TSCC) is the most frequent style of oral squamous cell carcinoma. However, the molecular mechanisms and function of LINC00961 in the TSCC progression remain unknown. In this study, we proved that LINC00961 expression was downregulated in TSCC cells (Tca8113, SCC1, SCC-4, and SCC-15) compared with normal tissue. In addition, we showed that LINC00961 expression was downregulated in TSCC samples compared with matched normal tissues. Moreover, ectopic expression of LINC00961 decreased TSCC cell growth and invasion and suppressed epithelial-mesenchymal transition in TSCC cell. Furthermore, we indicated that overexpression of LINC00961 decreased β-catenin expression. Knockdown of LINC00961 promoted cell proliferation and invasion partly via promoting the Wnt/β-catenin signaling pathway. These results suggested that LINC00961 was downregulated in TSCC tissues and acted as a tumor suppressor gene in the development of TSCC.
舌鳞状细胞癌（TSCC）是口腔鳞状细胞癌中最常见的类型。然而，LINC00961 在 TSCC 进展中的分子机制和功能尚不清楚。在本研究中，我们证明与正常组织相比，LINC00961 在 TSCC 细胞（Tca8113、SCC1、SCC-4 和 SCC-15）中的表达下调。此外，我们还表明与匹配的正常组织相比，LINC00961 在 TSCC 样本中的表达下调。此外，LINC00961 的异位表达降低了 TSCC 细胞的生长和侵袭，并抑制了 TSCC 细胞的上皮-间充质转化。此外，我们指出过表达 LINC00961 降低了 β-连环蛋白的表达。LINC00961 的敲低部分通过促进 Wnt/β-连环蛋白信号通路促进细胞增殖和侵袭。这些结果表明 LINC00961 在 TSCC 组织中下调，并在 TSCC 的发展中作为肿瘤抑制基因发挥作用。
