Doi Moeko, Sagawa Yuki, Sasano Kyohei, Tanaka Takumi, Mizutani Taeko, Okano Yuri, Masaki Hitoshi
Asahi-Ku, Osaka 535-0005, Japan (M.D., T.T.), Department of Bionics, Tokyo University of Technology Graduate School, Hachioji-shi 192-0982, Japan (Y.S., K.S., T.M., Y.O., H.M.).
J Cosmet Sci. 2019 Jan/Feb;70(1):17-31.
Recent increases in air pollution have raised concerns about its adverse effects on human health. Sacran is a natural polysaccharide isolated from a cyanobacterium. We previously reported that sacran improves skin conditions because of its effects as an artificial barrier against external stimuli, which suggested that sacran might protect the skin against air pollutants. The goal of this study was to characterize the potential of sacran to protect human skin against damage from air pollutants and to compare sacran with hyaluronic acid (HA). Sacran that was topically applied on the skin stayed on the surface or in the stratum corneum. Sacran-treated filters had a shielding effect against benzo[a]pyrene (BaP) and aldehyde compounds contained in tobacco smoke. Sacran suppressed the upregulation of cytochrome P4501A1 messenger ribonucleic acid (mRNA), which is a xenobiotic-metabolizing enzyme induced by BaP, and other responses against tobacco smoke in HaCaT keratinocytes. Furthermore, topical application of a serum containing 0.04% sacran on the skin reduced levels of carbonylated proteins in corneocytes of tobacco smokers. Sacran showed superior effects in every characteristic measured, compared with HA. We conclude that sacran ameliorates the oxidative stress initiated by tobacco smoke by shielding the skin surface and protects human skin.
近期空气污染的加剧引发了人们对其对人类健康不利影响的担忧。Sacran是一种从蓝藻中分离出的天然多糖。我们之前报道过，Sacran因其作为抵御外部刺激的人工屏障的作用而改善皮肤状况，这表明Sacran可能保护皮肤免受空气污染物的侵害。本研究的目的是表征Sacran保护人类皮肤免受空气污染物损害的潜力，并将Sacran与透明质酸（HA）进行比较。局部应用于皮肤的Sacran停留在皮肤表面或角质层中。经Sacran处理的滤器对香烟烟雾中含有的苯并[a]芘（BaP）和醛类化合物具有屏蔽作用。Sacran抑制了细胞色素P4501A1信使核糖核酸（mRNA）的上调，细胞色素P4501A1是一种由BaP诱导的外源性代谢酶，以及HaCaT角质形成细胞中对香烟烟雾的其他反应。此外，在皮肤上局部应用含0.04% Sacran的血清可降低吸烟者角质形成细胞中羰基化蛋白质的水平。与HA相比，Sacran在各项测量特征中均显示出更好的效果。我们得出结论，Sacran通过屏蔽皮肤表面减轻香烟烟雾引发的氧化应激，从而保护人类皮肤。
