Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Biosens Bioelectron. 2019 May 1;132:84-89. doi: 10.1016/j.bios.2019.02.038. Epub 2019 Feb 21.
A biosensor based on a chiral assembly of polymer of gold nanoparticle (AuNP) trimers was developed for the detection and quantification of the major shellfish allergen tropomyosin (TROP). TROP and anti-TROP monoclonal antibodies (mAb) were immobilized on 20 nm and 30 nm 16-mercaptohexadecanoic acid (16-MHDA) functionalized AuNPs to assemble a trimer, which has a Circular dichroism (CD) signal. The free TROP from samples was quantified as an inhibitor for the formation of the AuNP trimer. The AuNP trimer-based biosensor allowed for the selective determination of TROP in the range of 0.1-15 ng mL with the limit of detection (LOD) of 21 pg mL (S/N = 3) and the limit of quantitation (LOQ) of 70 pg mL (S/N = 10). The use of a AuNP trimer-based biosensor with simple sample preparation functions with specificity and accuracy; this highlights its applicability for the detection of allergens in shellfish products, related products and their production lines. Furthermore, based on the less conserved sequences of TROP in phylogenetically different species, this biosensor is currently being used to identify the adulteration of shellfish products using TROP as biomarker.
基于金纳米粒子（AuNP）三聚体手性组装的生物传感器被开发出来，用于检测和定量主要的贝类过敏原肌球蛋白（TROP）。TROP 和抗 TROP 单克隆抗体（mAb）被固定在 20nm 和 30nm 的 16-巯基十六烷酸（16-MHDA）功能化 AuNP 上来组装三聚体，它具有圆二色性（CD）信号。从样品中游离的 TROP 被定量为形成 AuNP 三聚体的抑制剂。基于 AuNP 三聚体的生物传感器允许在 0.1-15ng/mL 的范围内选择性地测定 TROP，检测限（LOD）为 21pg/mL（S/N=3），定量限（LOQ）为 70pg/mL（S/N=10）。该生物传感器具有简单的样品制备功能，特异性和准确性高；这突出了其在贝类产品、相关产品及其生产线中过敏原检测的适用性。此外，基于不同进化物种中 TROP 较少保守的序列，该生物传感器目前正被用于利用 TROP 作为生物标志物来识别贝类产品的掺假。
