Department of Mechanical Engineering, Indian Institute of Technology Delhi, New Delhi 110016, India.
Department of Mechanical Engineering, Indian Institute of Technology Delhi, New Delhi 110016, India.
J Mech Behav Biomed Mater. 2019 Jun;94:54-62. doi: 10.1016/j.jmbbm.2019.02.010. Epub 2019 Feb 19.
Tissue engineering is a field which aims to regenerate damaged tissues by enhancing tissue growth through the porous architecture of the scaffolds which is desired to mimic the human cancellous bone. Mg-based scaffolds are gaining importance in the field of tissue engineering owing to its potential application as a biomaterial. However, fabrication of porous Mg remains a daunting task due to its highly reactive nature. In the present work, a novel Mg-based open cell porous structure with pore interconnectivity and significant strength is successfully fabricated using powder metallurgy approach and Ti-woven wire mesh as a space holding material. Pore morphology and percentage porosity can be easily altered by adjusting the Ti-wire diameter and shape of construct. SEM, EDX and µ-CT analysis were performed to assess the microstructural properties of the fabricated scaffold which revealed a uniform distribution of pores with porosity varying in range 50-60%. The measured values of ultimate compressive strength and elastic modulus using quasi static compression test were found to be 101 MPa and 2 GPa, respectively. Further to improve corrosion resistance of fabricated scaffold, alloying and coating were carried out. Preliminary degradation study as well as cytocompatibility studies using L929 cells was carried out to validate the potential of fabricated scaffold for bone healing/repair applications. Fabricated porous structures showed improved corrosion resistance as well as cell viability of more than 90%, suggesting it as a promising development for bone scaffolding applications in future.
组织工程旨在通过增强组织生长来再生受损组织，其方法是通过模仿人体松质骨的多孔支架结构来增强组织生长。由于具有作为生物材料的潜在应用，基于镁的支架在组织工程领域越来越受到重视。然而，由于其高反应性，制造多孔镁仍然是一项艰巨的任务。在本工作中，使用粉末冶金方法和 Ti 编织线网作为空间保持材料成功制造了具有连通孔和显著强度的新型基于镁的开孔多孔结构。通过调整 Ti 线直径和结构形状，可以轻松改变孔形态和孔隙率。通过 SEM、EDX 和 µ-CT 分析评估了所制备支架的微观结构特性，结果表明具有均匀分布的孔，孔隙率在 50-60%范围内变化。使用准静态压缩试验测量的抗压强度和弹性模量的实测值分别为 101 MPa 和 2 GPa。为了进一步提高所制备支架的耐腐蚀性，进行了合金化和涂层处理。进行了初步降解研究以及使用 L929 细胞进行细胞相容性研究，以验证所制备支架在骨愈合/修复应用中的潜力。所制备的多孔结构显示出改善的耐腐蚀性和超过 90%的细胞活力，表明其有望成为未来骨支架应用的有前途的发展。
