Wistar大鼠中雄甾酮UDP-葡萄糖醛酸基转移酶活性的遗传缺陷是由于酶蛋白的缺失。

Genetic deficiency of androsterone UDP-glucuronosyltransferase activity in Wistar rats is due to the loss of enzyme protein.

作者信息

Matsui M, Nagai F

出版信息

Biochem J. 1986 Feb 15;234(1):139-44. doi: 10.1042/bj2340139.

DOI:10.1042/bj2340139

PMID:3085657

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1146536/

Abstract

Hepatic microsomal UDP-glucuronosyltransferases towards androsterone and testosterone were purified by chromatofocusing and UDP-hexanolamine affinity chromatography in Wistar rats which had genetic deficiency of androsterone UDP-glucuronosyltransferase activity. In rats with the high-activity phenotype, androsterone (the 3-hydroxy androgen) UDP-glucuronosyltransferase was eluted at about pH 7.4 and had a subunit Mr of 52 000, whereas testosterone (the 17-hydroxy steroid) UDP-glucuronosyltransferase was eluted at about pH 8.4 and had a subunit Mr of 50 000. The transferase that conjugates both androsterone and testosterone was eluted at about pH 8.0, had subunit Mr values of 50 000 and 52 000, and appeared to be an aggregate or hybrid of androsterone and testosterone UDP-glucuronosyltransferases. In rats with the low-activity phenotype, androsterone UDP-glucuronosyltransferase was absent, whereas testosterone UDP-glucuronosyltransferase was eluted at around pH 8.5, with a subunit Mr of 50 000.

摘要

采用色谱聚焦法和UDP - 己醇胺亲和色谱法，对雄甾酮UDP - 葡萄糖醛酸基转移酶活性存在基因缺陷的Wistar大鼠肝微粒体中针对雄甾酮和睾酮的UDP - 葡萄糖醛酸基转移酶进行了纯化。在具有高活性表型的大鼠中，雄甾酮（3 - 羟基雄激素）UDP - 葡萄糖醛酸基转移酶在pH约7.4处洗脱，亚基分子量为52000，而睾酮（17 - 羟基类固醇）UDP - 葡萄糖醛酸基转移酶在pH约8.4处洗脱，亚基分子量为50000。同时结合雄甾酮和睾酮的转移酶在pH约8.0处洗脱，亚基分子量值为50000和52000，似乎是雄甾酮和睾酮UDP - 葡萄糖醛酸基转移酶的聚集体或杂合体。在具有低活性表型的大鼠中，不存在雄甾酮UDP - 葡萄糖醛酸基转移酶，而睾酮UDP - 葡萄糖醛酸基转移酶在pH约8.5处洗脱，亚基分子量为50000。