Pujirahayu Niken, Suzuki Toshisada, Katayama Takeshi
Laboratory of Biomass Chemistry, Faculty of Agriculture, Kagawa University, Kagawa 761-0795, Japan.
Department of Forestry, Faculty of Forestry and Environmental Sciences, Halu Oleo University, Kendari 93232, Indonesia.
Plants (Basel). 2019 Mar 8;8(3):57. doi: 10.3390/plants8030057.
This study clarifies the chemical constituents and botanical origin of Cockerell bee propolis collected from Southeast Sulawesi, Indonesia. Propolis samples and resin of were extracted with 99% ethanol to obtain an ethanol extract of propolis (EEP) and an ethanol extract of resin (EEM). Column chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC) were developed and used for the separation and isolation of compounds from the ether-soluble fraction. The structure of the compounds was determined by nuclear magnetic resonance (NMR) spectroscopic analysis, and their molecular weight analyzed by gas chromatography⁻mass spectrometry (GC⁻MS). The HPLC chromatogram of the EEP was then compared with the HPLC chromatogram of EEM to investigate the botanical origin of propolis. Five compounds were isolated from the EEP, and their structures were determined as mangiferolic acid, cycloartenol, ambonic acid, mangiferonic acid, and ambolic acid, which are cycloartane-type triterpenes. The characteristic peak of the HPLC chromatograms of EEP and EEM showed a similar pattern, which is that the main components of propolis were also found in resin. These results suggested that the propolis from Southeast Sulawesi was rich in cycloartane-type triterpenes, and the plant source of the propolis could be (mango).
本研究阐明了从印度尼西亚东南苏拉威西采集的科克雷尔蜂胶的化学成分和植物来源。用99%乙醇提取蜂胶样品和树脂，得到蜂胶乙醇提取物（EEP）和树脂乙醇提取物（EEM）。采用柱色谱、薄层色谱（TLC）和高效液相色谱（HPLC）从醚溶性部分分离和纯化化合物。通过核磁共振（NMR）光谱分析确定化合物的结构，并用气相色谱-质谱联用（GC-MS）分析其分子量。然后将EEP的HPLC色谱图与EEM的HPLC色谱图进行比较，以研究蜂胶的植物来源。从EEP中分离出5种化合物，其结构确定为芒果醇酸、环阿屯醇、安波酸、芒果酮酸和安波醇酸，均为环阿尔廷型三萜。EEP和EEM的HPLC色谱图的特征峰显示出相似的模式，即蜂胶的主要成分在树脂中也有发现。这些结果表明，东南苏拉威西的蜂胶富含环阿尔廷型三萜，其植物来源可能是芒果。
