School of Life Sciences, Zhengzhou University, Henan, PR China.
School of Life Sciences, Zhengzhou University, Henan, PR China.
Int J Biol Macromol. 2019 Jun 15;131:323-328. doi: 10.1016/j.ijbiomac.2019.03.056. Epub 2019 Mar 8.
In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL). The process variables were optimized by the isovariant central composite design to improve the pectin extraction yield. The optimum conditions obtained were as follows, extraction time 2 h, temperature 90 °C, pH 2 and liquid-solid (LS) ratio 50 mL/g. The extraction yield was 18.25% under these conditions, which was close to the predicted value (17.60%). Then the pectin was characterized by gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods. The galacturonic acid content was 82.75%, and on this basis, the other monosaccharide composition analysis illustrated that pectin from PMTL was also composed of rhamnose, arabinose, mannose, glucose and galactose in a ratio of 2.96:1.17:1.04:8.07:2.05. The pectin of PMTL had an esterification degree of 62.50% and it showed good antioxidant activity. Taken together, the pectin of PMTL could be used as potential additive in food industry.
在这项工作中，响应面法（RSM）和微波预处理被用于从紫珠属植物叶片中提取果胶。通过等变异中心复合设计优化了工艺变量，以提高果胶的提取率。得到的最佳条件如下：提取时间 2 小时，温度 90°C，pH 2 和液固比（LS）为 50 mL/g。在此条件下，提取率为 18.25%，接近预测值（17.60%）。然后用气相色谱（GC）、分光光度法（UV-可见分光光度法）和光谱法（傅里叶变换红外光谱法）对果胶进行了表征。半乳糖醛酸含量为 82.75%，在此基础上，其他单糖组成分析表明，PMTL 果胶还由鼠李糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖以 2.96:1.17:1.04:8.07:2.05 的比例组成。PMTL 果胶的酯化度为 62.50%，具有良好的抗氧化活性。综上所述，PMTL 果胶可用作食品工业的潜在添加剂。
