Programa de Pós-Graduação em Engenharia e Ciência de Alimentos, Escola de Química e Alimentos, Laboratório de Micotoxinas e Ciência de Alimentos, Universidade Federal do Rio Grande (FURG), Avenida Itália, km 8, Bairro Carreiros, Rio Grande, RS, CEP: 96203-900, Brazil.
Programa de Pós-Graduação em Bioquímica e Bioprospecção, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Laboratório de Controle de Contaminantes em Biomateriais, Universidade Federal de Pelotas (UFPel), Avenida Eliseu Maciel, S/N, Capão do Leão, Pelotas, RS, CEP: 96160-000, Brazil.
Curr Microbiol. 2019 Aug;76(8):930-938. doi: 10.1007/s00284-019-01663-2. Epub 2019 Mar 11.
Phenolic (free, conjugated and bound) and carotenoid extracts from microalgae Nannochloropsis sp. and Spirulina sp. were investigated regarding their potential to mitigate contamination by Fusarium complex fungal pathogens. Free phenolic acid extracts from both microalgae were the most efficient, promoting the lowest mycelial growth rates of 0.51 cm day (Spirulina sp.) and 0.78 cm day (Nannochloropsis sp.). An experiment involving natural free phenolic acid extracts and synthetic solutions was carried out based on the natural phenolic acid profile. The results revealed that the synthetic mixtures of phenolic acids from both microalgae were less efficient than the natural extracts at inhibiting fungal growth, indicating that no purification is required. The half-maximal effective concentration (EC) values of 49.6 μg mL and 33.9 μg mL were determined for the Nannochloropsis and Spirulina phenolic acid extracts, respectively. The use of phenolic extracts represents a new perspective regarding the application of compounds produced by marine biotechnology to prevent Fusarium species contamination.
从微藻 Nannochloropsis sp. 和螺旋藻 sp. 中提取的酚类（游离、结合和结合）和类胡萝卜素提取物，就其减轻镰刀菌复合真菌病原体污染的潜力进行了研究。两种微藻的游离酚酸提取物最为有效，促进了最低的菌丝生长速率，分别为 0.51 cm 天（螺旋藻）和 0.78 cm 天（Nannochloropsis sp.）。基于天然酚酸图谱进行了涉及天然游离酚酸提取物和合成溶液的实验。结果表明，来自两种微藻的合成混合酚酸的混合物在抑制真菌生长方面不如天然提取物有效，表明不需要纯化。Nannochloropsis 和螺旋藻酚酸提取物的半最大有效浓度 (EC) 值分别为 49.6 μg mL 和 33.9 μg mL。酚类提取物的使用为利用海洋生物技术生产的化合物来防止镰刀菌属污染提供了一个新的视角。
