Spyridon Ntougias, Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100, Xanthi, Greece.
Environ Sci Pollut Res Int. 2019 May;26(14):14238-14249. doi: 10.1007/s11356-019-04771-5. Epub 2019 Mar 12.
An enrichment process was employed by applying high ibuprofen concentration in an immobilized cell bioreactor in order to favor the ibuprofen-degrading community present in activated sludge. Experimental data showed the ability of the immobilized cell bioreactor to achieve high ibuprofen removal efficiencies (98.4 ± 0.3%), the tendency of the enriched biomass to acidify the treated liquor, and the inhibition of the nitrification process. Illumina sequencing revealed a massive increase in the relative abundance of Alphaproteobacteria and Gammaproteobacteria (from 29.1 to 80.8%) and a dramatic decrease in the proportion of Bacteroidetes, Planctomycetes, and Verrucomicrobia (from 42.7 to 2.1%) when pure ibuprofen served as the sole carbonaceous feeding substrate. This shift in the feeding conditions resulted in the predominance of Novosphingobium and Rhodanobacter (25.5 ± 10.8% and 25.2 ± 3.0%, respectively) and demonstrated a specialized ibuprofen-degrading bacterial community in activated sludge, which possessed the selective advantage to cope with its degradation. To the best of our knowledge, this bioreactor system was capable of effectively treating the highest ibuprofen concentration applied in wastewater treatment plants.
采用固定化细胞生物反应器并施加高浓度布洛芬的方法来富集，以利于存在于活性污泥中的布洛芬降解菌群。实验数据表明，固定化细胞生物反应器具有实现高布洛芬去除效率（98.4±0.3%）的能力，富集生物量使处理液酸化的趋势，以及对硝化过程的抑制作用。Illumina 测序显示，当纯布洛芬作为唯一的碳源时，α变形菌门和γ变形菌门的相对丰度大量增加（从 29.1%增加到 80.8%），而拟杆菌门、浮霉菌门和疣微菌门的比例急剧下降（从 42.7%下降到 2.1%）。这种喂养条件的改变导致了新鞘氨醇单胞菌和罗得河菌（分别为 25.5±10.8%和 25.2±3.0%）的优势地位，并证明了活性污泥中存在具有特殊功能的布洛芬降解细菌群落，该群落具有降解布洛芬的选择性优势。据我们所知，该生物反应器系统能够有效地处理在污水处理厂应用的最高布洛芬浓度。
