Viedma J A, de la Iglesia A, Parera M, López M T
Clin Chem. 1986 Jun;32(6):1020-2.
This rapid, sensitive equilibrium turbidimetric immunoassay for quantification of alpha 1-antitrypsin involves a monospecific antibody, polyethylene glycol 6000 to accelerate and enhance the immunoprecipitation reaction, and Tween 20 surfactant to decrease and stabilize the sample-blank values. Turbidity at 334 nm is measured by an automated discrete analyzer. Grossly lipemic, icteric, or hemolyzed samples can be assayed. Correlation with results by radial immunodiffusion (RID) was excellent (r = 0.97, n = 84). Analytical recovery averaged 97.7 (SD 2.9)%. Within-run CVs ranged from 1.6 to 1.9%, between-day CVs from 2.0 to 3.5%. Reference values for healthy adults (n = 147) were determined by parametric estimation (for an assumed normal distribution of untransformed data). The lower limit (g/L) with its 0.90 confidence interval is 1.23 (range 1.18-1.28), the upper limit is 2.15 (2.10-2.20), and the mean is 1.69 g/L.
这种用于定量α1-抗胰蛋白酶的快速、灵敏的平衡比浊免疫分析方法涉及一种单特异性抗体、聚乙二醇6000以加速和增强免疫沉淀反应，以及吐温20表面活性剂以降低并稳定样品空白值。通过自动离散分析仪测量334nm处的吸光度。严重脂血、黄疸或溶血的样品均可进行检测。与放射免疫扩散法（RID）结果的相关性良好（r = 0.97，n = 84）。分析回收率平均为97.7（标准差2.9）%。批内变异系数范围为1.6%至1.9%，批间变异系数为2.0%至3.5%。通过参数估计（针对未转换数据的假定正态分布）确定了健康成年人（n = 147）的参考值。下限（g/L）及其90%置信区间为1.23（范围1.18 - 1.28），上限为2.15（2.10 - 2.20），平均值为1.69g/L。
