Otsuji S, Shibata H, Umeda M
Clin Chem. 1982 Oct;28(10):2121-4.
This rapid, reliable equilibrium turbidimetric immunoassay for serum C-reactive protein involves a potent monospecific antibody. Polyethylene glycol-6000 to accelerate and enhance the immunoprecipitation reaction, and Tween-20 surfactant to lower and stabilize the sample blank values. Grossly lipemic, icteric, or hemolyzed sera can be assayed. Values up to about 220 mg/L, for which the standard curve is linear, can be measured without sample dilution. Results by the proposed method and by radial immunodiffusion (r 0.989) or laser nephelometry (r = 0.957) correlated well. Analytical recovery averaged 101.3%. Within-, between-, and day-to-day CVs ranged from 0.9% to 3.5%, 0.8% to 5.5%, and 1.9% to 4.8%, respectively. The method is demonstrably superior to radial immunodiffusion or nephelometry. Any spectrophotometer that can measure turbidimetrically at 340 nm can be used.
这种用于血清C反应蛋白的快速、可靠的平衡比浊免疫测定法涉及一种高效的单特异性抗体。使用聚乙二醇6000来加速和增强免疫沉淀反应，以及吐温-20表面活性剂来降低并稳定样品空白值。严重脂血、黄疸或溶血的血清均可进行检测。对于标准曲线呈线性的高达约220mg/L的值，无需样品稀释即可测量。所提出的方法与放射免疫扩散法（r = 0.989）或激光散射比浊法（r = 0.957）的结果相关性良好。分析回收率平均为101.3%。批内、批间和日间变异系数分别为0.9%至3.5%、0.8%至5.5%和1.9%至4.8%。该方法明显优于放射免疫扩散法或散射比浊法。任何能够在340nm处进行比浊测量的分光光度计均可使用。
