a Department of Cardiology , Hainan Western Central Hospital , Danzhou , China.
b Department of Cardiac Surgery , Zibo Central Hospital , Zibo , China.
Pharm Biol. 2019 Dec;57(1):176-183. doi: 10.1080/13880209.2019.1577467.
Ophiopogonis Radix, the root of Ophiopogon japonicus (Thunb.) Ker-Gawl (Liliaceae), is a Traditional Chinese Medicine, which has been investigated to possess effective treatment of cardiovascular diseases.
This study evaluates the cardioprotective effects of steroidal saponins extract from Ophiopogon japonicus (SOJ) root against doxorubicin-induced chronic heart failure (CHF) through the amelioration of oxidative stress and inflammation.
A Sprague-Dawley rat model of CHF was established by intraperitoneally injected with DOX. All rats were randomly divided into four groups: Control group, CHF group, CHF + SOJ (100 mg/kg) treatment group, SOJ (100 mg/kg) treatment group (n = 8/group). After six weeks administration, biometric and echocardiography were measured. The levels of biochemical parameters were measured using commercial kits.
The values of LVESP, +dP/dtmax, -dP/dtmax, EF and FS increased to 116.20 ± 1.68 mmHg, 2978.71 ± 168.26 mmHg/s, 3452.61 ± 286.09 mmHg/s, 68.26 ± 5.28% and 31.97 ± 3.79%, respectively; the values of LVEDP, LVESD and LVEDD decreased to 8.85 ± 0.84 mmHg, 8.39 ± 0.45 mm and 12.36 ± 0.87 mm in CHF + SOJ group. In addition, the levels of IL-6, TNF-α and IL-1β decreased to 154.41 ± 7.72 pg/mg protein, 110.02 ± 6.96 pg/mg protein and 39.39 ± 5.27 pg/mg protein, respectively; the relative activity of p38 MAPK decreased to 2.60 ± 0.40 in CHF + SOJ group. Furthermore, the activities of SOD, CAT and GSH-Px increased to 268.77 ± 6.20 U/mg protein, 13.68 ± 0.68 U/mg protein and 316.90 ± 8.08 µmol/mg protein, and the content of MDA decreased to 4.03 ± 0.43 nmol/mg protein in CHF + SOJ group.
SOJ exerts the cardioprotective effect against DOX-induced CHF through suppressing inflammatory and oxidative stress. These results provide evidence that SOJ might be an effective treatment for CHF.
麦冬，是百合科沿阶草属的多年生常绿草本植物，是一种中药，已被证明对心血管疾病有有效的治疗作用。
本研究通过改善氧化应激和炎症，评估来自麦冬根的甾体皂甙提取物（SOJ）对阿霉素诱导的慢性心力衰竭（CHF）的心脏保护作用。
通过腹腔注射多柔比星（DOX）建立 Sprague-Dawley 大鼠 CHF 模型。所有大鼠随机分为四组：对照组、CHF 组、CHF+SOJ（100mg/kg）治疗组、SOJ（100mg/kg）治疗组（n=8/组）。给药 6 周后，测量生物计量学和超声心动图。使用商业试剂盒测量生化参数的水平。
LVESP、+dP/dtmax、-dP/dtmax、EF 和 FS 值分别增加到 116.20±1.68mmHg、2978.71±168.26mmHg/s、3452.61±286.09mmHg/s、68.26±5.28%和 31.97±3.79%；LVEDP、LVESD 和 LVEDD 值分别降低至 8.85±0.84mmHg、8.39±0.45mm 和 12.36±0.87mm，在 CHF+SOJ 组。此外，IL-6、TNF-α 和 IL-1β 的水平分别降低至 154.41±7.72pg/mg 蛋白、110.02±6.96pg/mg 蛋白和 39.39±5.27pg/mg 蛋白；p38 MAPK 的相对活性降低至 2.60±0.40，在 CHF+SOJ 组。此外，SOJ 组 SOD、CAT 和 GSH-Px 的活性分别增加至 268.77±6.20U/mg 蛋白、13.68±0.68U/mg 蛋白和 316.90±8.08µmol/mg 蛋白，MDA 含量降低至 4.03±0.43nmol/mg 蛋白，在 CHF+SOJ 组。
SOJ 通过抑制炎症和氧化应激对 DOX 诱导的 CHF 发挥心脏保护作用。这些结果为 SOJ 可能是 CHF 的有效治疗方法提供了证据。
