School of Engineering, Institute for Integrated Micro and Nanosystems, University of Edinburgh, Edinburgh, UK.
Electrophoresis. 2019 Sep;40(18-19):2575-2583. doi: 10.1002/elps.201900057. Epub 2019 Mar 19.
Dielectrophoresis (DEP) studies have progressed from the microscopic scale of cells and bacteria, through the mesoscale of virions to the molecular scale of DNA and proteins. The Clausius-Mossotti function, based on macroscopic electrostatics, is invariably employed in the analyses of all these studies. The limitations of this practice are explored, with the conclusion that it should be abandoned for the DEP study of proteins and modified for native DNA. For macromolecular samples in general, a DEP theory that incorporates molecular-scale interactions and the influence of permanent dipoles is more appropriate. Experimental ways to test these conclusions are proposed.
电泳（DEP）的研究已经从细胞和细菌的微观尺度，通过病毒的介观尺度，发展到 DNA 和蛋白质的分子尺度。基于宏观静电学的克劳修斯-莫索蒂函数，在所有这些研究的分析中都始终得到应用。本文探讨了这种做法的局限性，得出的结论是，对于蛋白质的 DEP 研究，应该放弃这种做法，并对天然 DNA 进行修正。对于一般的高分子样品，更适合采用包含分子尺度相互作用和永久偶极子影响的 DEP 理论。本文提出了一些实验方法来验证这些结论。
