Fuchs J-G, Moënne-Loccoz Y, Défago G
Institute for Plant Sciences/Phytopathology, Swiss Federal Institute of Technology (ETH Zentrum), Universitätstr. 2, CH-8092 Zürich, Switzerland.
Plant Dis. 1997 May;81(5):492-496. doi: 10.1094/PDIS.1997.81.5.492.
Nonpathogenic Fusarium oxysporum strain Fo47 controls the incidence of Fusarium wilt. Four bioassays in which a strain of the pathogen F. oxysporum f. sp. lycopersici and Fo47 were not in direct contact were developed to evaluate whether Fo47 could induce resistance to Fusarium wilt in tomato plants. Fo47 and the pathogen were separated either physically or in time. Bio-assays were carried out under hydroponic conditions (two bioassays), in potting mix, or in autoclaved soil. Strain Fo47 protected tomato against Fusarium wilt in all four bioassays. Inoculation with Fo47 increased chitinase, β-1,3-glucanase, and β-1,4-glucosidase activity in plants, confirming the ability of Fo47 to induce resistance in tomato. This report is the first to demonstrate that a nonpathogenic strain of F. oxysporum can induce resistance to Fusarium wilt in tomato plants. This result has important practical implications for biocontrol of tomato diseases under commercial conditions.
非致病性尖孢镰刀菌菌株Fo47可控制枯萎病的发病率。开展了四项生物测定，其中病原菌尖孢镰刀菌番茄专化型菌株与Fo47不直接接触，以评估Fo47是否能诱导番茄植株对枯萎病产生抗性。Fo47与病原菌在物理上或时间上相互分离。生物测定在水培条件下（两项生物测定）、盆栽混合料或经高压灭菌的土壤中进行。在所有四项生物测定中，菌株Fo47均保护番茄免受枯萎病侵害。用Fo47接种可提高植物中几丁质酶、β-1,3-葡聚糖酶和β-1,4-葡糖苷酶的活性，证实了Fo47诱导番茄产生抗性的能力。本报告首次证明非致病性尖孢镰刀菌菌株可诱导番茄植株对枯萎病产生抗性。该结果对商业条件下番茄病害的生物防治具有重要的实际意义。
