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菌株 Fo47 的染色体级基因组组装,一种真菌内生菌和生防剂。

Chromosome-Scale Genome Assembly of Strain Fo47, a Fungal Endophyte and Biocontrol Agent.

机构信息

MOE Key Laboratory for Intelligent Networks & Network Security, Faculty of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

Graduate Program of Plant Biology, University of Massachusetts Amherst, Amherst, MA 01003, U.S.A.

出版信息

Mol Plant Microbe Interact. 2020 Sep;33(9):1108-1111. doi: 10.1094/MPMI-05-20-0116-A. Epub 2020 Jul 28.

Abstract

Here, we report a chromosome-level genome assembly of Fo47 (12 pseudomolecules; contig N: 4.52 Mb), generated using a combination of PacBio long-read, Illumina paired end, and high-throughput chromosome conformation capture sequencing data. Although causes vascular wilt to over 100 plant species, the strain Fo47 is classified as an endophyte and is widely used as a biocontrol agent for plant disease control. The Fo47 genome carries a single accessory chromosome of 4.23 Mb, compared with the reference genome of f. sp. Fol4287. The high-quality assembly and annotation of the Fo47 genome will be a valuable resource for studying the mechanisms underlying the endophytic interactions between and plants as well as for deciphering the genome evolution of the species complex.

摘要

在这里,我们报告了 Fo47(12 个假染色体;N 号染色体:4.52Mb)的染色体水平基因组组装,该组装使用 PacBio 长读长、Illumina 配对末端和高通量染色体构象捕获测序数据的组合生成。尽管 Fo47 菌株可以引起超过 100 种植物物种的血管萎蔫病,但它被归类为内生菌,并被广泛用作植物病害防治的生物防治剂。Fo47 基因组携带一个 4.23Mb 的单一辅助染色体,而参考基因组 f. sp. Fol4287 则没有。高质量的 Fo47 基因组组装和注释将是研究 Fo47 与植物之间内生相互作用机制以及破译 Fo47 种复合体基因组进化的宝贵资源。

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