Nugent Brian, Ali Shahin S, Mullins Ewen, Doohan Fiona M
Molecular Plant-Microbe Interactions Laboratory, School of Biology and Environmental Science, University College Dublin, Dublin, Ireland.
Department of Crop Science, Teagasc Research Centre, Carlow, Ireland.
Front Microbiol. 2019 Feb 26;10:295. doi: 10.3389/fmicb.2019.00295. eCollection 2019.
is a leading microbial agent in the emerging consolidated bioprocessing (CBP) industry owing to its capability to infiltrate the plant's lignin barrier and degrade complex carbohydrates to value-added chemicals such as bioethanol in a single step. Membrane transport of nutrients is a key factor in successful microbial colonization of host tissue. This study assessed the impact of a peptide transporter on ability to convert lignocellulosic straw to ethanol. We characterized a novel peptide transporter () of the dipeptide/tripeptide transporter (PTR) class. represents a novel transporter with high homology to the peptide transporters and . Its expression level was highly activated in nitrogen-poor environments, which is a characteristic of PTR class peptide transporters. Overexpression and post-translational gene silencing of the in affected the peptide transport capacity and ethanol yielded from a both a wheat straw/bran mix and glucose. Thus, we conclude that it plays a role in the nutrient acquisition system of which serves to not only enhance fungal fitness but also CBP efficacy.
由于其能够渗透植物的木质素屏障并将复杂碳水化合物一步降解为增值化学品（如生物乙醇），在新兴的整合生物加工（CBP）行业中是一种主要的微生物制剂。营养物质的膜转运是宿主组织成功进行微生物定殖的关键因素。本研究评估了一种肽转运蛋白对将木质纤维素秸秆转化为乙醇能力的影响。我们鉴定了一种二肽/三肽转运蛋白（PTR）类的新型肽转运蛋白（）。代表了一种与肽转运蛋白和具有高度同源性的新型转运蛋白。其表达水平在贫氮环境中高度激活，这是PTR类肽转运蛋白的一个特征。在中对进行过表达和翻译后基因沉默影响了肽转运能力以及从小麦秸秆/麸皮混合物和葡萄糖中产生的乙醇。因此，我们得出结论，它在的营养获取系统中起作用，这不仅有助于提高真菌适应性，还能提高CBP效率。
